Rabbit Anti-CDC37 Recombinant Antibody (clone 10C3) (CAT#: ZG-0605J)
This product is a rabbit antibody that recognizes CDC37.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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WB
Figure 1 Rabbit Anti-CDC37 Antibody (ZG-0605J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, PC-3 whole cell lysate
All lanes: CDC37 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 45 kDa
Observed band size: 50 kDa
FC
Figure 2 Rabbit Anti-CDC37 Antibody (ZG-0605J) in FC
Overlay histogram showing Hela cells stained with ZG-0605J (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.
Specifications
- Immunogen
- A synthesized peptide derived from human Cdc37
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CDC37
- Species Reactivity
- Human
- Clone
- 10C3
- Applications
- ELISA, WB, FC
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry.
WB:1:500-1:5000, FC:1:20-1:200
Target
- Alternative Names
- P50CDC37; hsp90 co-chaperone Cdc37; CDC37 (cell division cycle 37, S. cerevisiae, homolog); CDC37 cell division cycle 37 homolog; cell division cycle 37 homolog; hsp90 chaperone protein kinase-targeting subunit
- Gene ID
- 11140
- UniProt ID
- Q16543
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibodies
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2189z | Mouse Anti-CDC37 Recombinant Antibody (clone 16H3) | WB, IF, ELISA | Mouse IgG2a |
| MRO-0328-CN | Rabbit Anti-CDC37 Recombinant Antibody (clone CBACN-129) | WB, IHC, IP, FC | Rabbit IgG |
| VS3-FY329 | Recombinant Rabbit Anti-CDC37 Antibody (clone R04-0H4) | WB, IP | Rabbit IgG |
| VS3-FY330 | Recombinant Rabbit Anti-CDC37 Antibody (clone R04-4I8) | WB, IP | Rabbit IgG |
| VS7-HM663 | Mouse Anti-CDC37 Recombinant Antibody (clone 6B3B7) | WB, FCM | Mouse IgG2a |
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For Research Use Only. Not For Clinical Use.
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