This product is antibody recognizes CDH17. The antibody D2101 can be used in some immunoassay techniques such as: ELISA.
Figure 1 Interaction of D2101 with EC1 and EC12 from human and mouse CDH17 analyzed by surface plasmon resonance.
Human EC1: red line, human EC12: blue line, mouse EC1: orange line, and mouse EC12: green line
Fujiwara, K., Tsuji, A. B., Sudo, H., Sugyo, A., Akiba, H., Iwanari, H.,... & Higashi, T. (2019). 111 In-labeled anti-cadherin17 antibody D2101 has potential as a noninvasive imaging probe for diagnosing gastric cancer and lymph-node metastasis. Annals of nuclear medicine, 1-11.
Figure 2 ELISA of intact D2101 and DTPA-D2101 using gastric cancer AGS cells. Intact anti-CDH17 antibody D2101: open circles, DTPA-D2101: filled circles. (n = 2 per each point)
Fujiwara, K., Tsuji, A. B., Sudo, H., Sugyo, A., Akiba, H., Iwanari, H.,... & Higashi, T. (2019). 111 In-labeled anti-cadherin17 antibody D2101 has potential as a noninvasive imaging probe for diagnosing gastric cancer and lymph-node metastasis. Annals of nuclear medicine, 1-11.
Figure 3 Serial SPECT/CT images of AGS xenograft mice using the ¹¹¹In-labeled anti-CDH17 antibody D2101.
Upper panels: coronal images; lower panels: transaxial images. Arrowheads indicate tumors
Fujiwara, K., Tsuji, A. B., Sudo, H., Sugyo, A., Akiba, H., Iwanari, H.,... & Higashi, T. (2019). 111 In-labeled anti-cadherin17 antibody D2101 has potential as a noninvasive imaging probe for diagnosing gastric cancer and lymph-node metastasis. Annals of nuclear medicine, 1-11.
Figure 4 Serial SPECT/CT images of MKN74 xenograft mice using the ¹¹¹In-labeled anti-CDH17 antibody D2101.
Upper panels: coronal images; lower panels: transaxial images. Arrowheads indicate tumors
Fujiwara, K., Tsuji, A. B., Sudo, H., Sugyo, A., Akiba, H., Iwanari, H.,... & Higashi, T. (2019). 111 In-labeled anti-cadherin17 antibody D2101 has potential as a noninvasive imaging probe for diagnosing gastric cancer and lymph-node metastasis. Annals of nuclear medicine, 1-11.
Figure 5 Specific binding to the endogenous CDH17 shown by immunoblot.
The left panel shows the Coomassie brilliant blue staining of the AGS cell lysate. The center panel shows the detection of the commercially available anti-CDH17 mAb, MAB1032. The right panel shows the detection of our in-house generated anti-CDH17 mAb, D2101.
Kusano-Arai, O., Iwanari, H., Kudo, S., Kikuchi, C., Yui, A., Akiba, H.,... & Hamakubo, T. (2018). Synergistic cytotoxic effect on gastric cancer cells of an immunotoxin cocktail in which antibodies recognize different epitopes on CDH17. Monoclonal antibodies in immunodiagnosis and immunotherapy, 37(1), 1-11.
Figure 6 Specific binding to the cell surface CDH17 was demonstrated by flow cytometry.
The filled histograms indicate anti-CDH17 mAbs, and the open histograms indicate control IgG binding to AGS cells (upper panels) and MKN74 cells (lower panels). The numbers at the top designate the clone number of the anti-CDH17 mAbs. CDH17, Cadherin-17; mAb, monoclonal antibody.
Kusano-Arai, O., Iwanari, H., Kudo, S., Kikuchi, C., Yui, A., Akiba, H.,... & Hamakubo, T. (2018). Synergistic cytotoxic effect on gastric cancer cells of an immunotoxin cocktail in which antibodies recognize different epitopes on CDH17. Monoclonal antibodies in immunodiagnosis and immunotherapy, 37(1), 1-11.
Figure 7 Dose-dependent binding of the anti-CDH17 mAbs.
All of the data shown are the means-standard deviations of triplicate assay.
Kusano-Arai, O., Iwanari, H., Kudo, S., Kikuchi, C., Yui, A., Akiba, H.,... & Hamakubo, T. (2018). Synergistic cytotoxic effect on gastric cancer cells of an immunotoxin cocktail in which antibodies recognize different epitopes on CDH17. Monoclonal antibodies in immunodiagnosis and immunotherapy, 37(1), 1-11.
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• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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CAT | Product Name | Application | Type |
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TAB-1415CL-S(P) | Mouse Anti-CDH17 Recombinant Antibody; scFv Fragment (TAB-1415CL-S(P)) | ELISA | Mouse scFv |
TAB-1416CL-S(P) | Mouse Anti-CDH17 Recombinant Antibody; scFv Fragment (TAB-1416CL-S(P)) | ELISA | Mouse scFv |
TAB-1417CL-S(P) | Mouse Anti-CDH17 Recombinant Antibody; scFv Fragment (TAB-1417CL-S(P)) | ELISA | Mouse scFv |
TAB-1418CL-S(P) | Mouse Anti-CDH17 Recombinant Antibody; scFv Fragment (TAB-1418CL-S(P)) | ELISA | Mouse scFv |
TAB-1419CL-S(P) | Mouse Anti-CDH17 Recombinant Antibody; scFv Fragment (TAB-1419CL-S(P)) | ELISA | Mouse scFv |
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(Creative Biolabs Cat# HPAB-2061LY, RRID: AB_3111424)
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