Rabbit Anti-CEACAM1 Recombinant Antibody (clone 10H8)
CAT#: ZG-0082U
The purified Rabbit Anti-CEACAM1 Recombinant Antibody (clone 10H8) displayed high specificity against the CEACAM1 protein of human.
Tested Data
Gene Expression
IHC
Figure 1 Rabbit Anti-CEACAM1 Antibody (ZG-0082U) in IHC
IHC image of ZG-0082U diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Normal Tissue
Figure 1 Colon
Enterocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2146/5911_A_8_3.jpg
Normal Tissue
Figure 2 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2146/5911_A_9_5.jpg
RNA Expression
Figure 3 RNA cell line category: Group enriched (CAPAN-2, Hep G2, OE19, RT4, SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000079385-CEACAM1
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Specifications
- Immunogen
- A synthesized peptide derived from human CEACAM1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CEACAM1
- Species Reactivity
- Human
- Clone
- 10H8
- Applications
- ELISA, IHC
- Conjugate
- Unconjugated
- Related Disease
- Acute Gonococcal Endometritis and Adenomatoid Tumor
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunohistochemistry. Specific conditions should be optimized by the end user. IHC:1:50-1:200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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