Rabbit Anti-CHEK1 (pS296) Recombinant Antibody (clone R02-4Z6) (CAT#: VS3-WK2139)
This product is a rabbit recombinant antibody that is specific to phosphorylated CHEK1. The target of this antibody is phospho-CHEK1 (Ser296). The clone R02-4Z6 can be used in WB analysis at a dilution of 1/500-1/1000. The molecular weight of CHEK1 (pSer296) observed in WB experiments using this antibody is 54 kDa.
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Custom Production
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Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44364/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44364/if_selected.jpg
Figure 2 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44364/804_D1_1_red_green.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44364/804_D1_1_red_green.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44364/712_D1_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/44364/712_D1_1_red_green.jpg
Figure 2 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000149554-CHEK1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000149554-CHEK1
Figure 4 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000149554-CHEK1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000149554-CHEK1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Ser296 of human CHEK1
- Species Reactivity
- Human
- Clone
- R02-4Z6
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
Target
- Alternative Names
- Checkpoint Kinase 1; Cell Cycle Checkpoint Kinase; CHK1 Checkpoint Homolog; EC 2.7.11.1; CHK1; Serine/Threonine-Protein Kinase Chk1; Checkpoint, S. Pombe, Homolog Of, 1
- Gene ID
- 1111
- UniProt ID
- O14757
- Long Name
- Checkpoint Kinase 1
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0005CT | Recombinant Mouse anti-Human CHEK1 Monoclonal antibody (7G6) | WB | |
| MRO-0359-CN | Rabbit Anti-CHEK1 Recombinant Antibody (clone CBACN-146) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0016U | Rabbit Anti-CHEK1 Recombinant Antibody (clone 2F2) | ELISA, WB, IHC, IF | Rabbit IgG |
| VS3-CJ127 | Rabbit Anti-CHEK1 Recombinant Antibody (VS3-CJ127) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| VS3-XY3314 | Rabbit Anti-CHEK1 Recombinant Antibody (clone R02-1D6) | WB, ICC, IF, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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