AbPlus™ Anti-CHEK2 Magnetic Beads (VS-0724-YC455)
CAT#: VS-0724-YC455
The AbPlus Anti-CHEK2 Magnetic Beads (VS-0724-YC455) is an innovative affinity resin which is bound with anti-CHEK2 specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of CHEK2 protein under native and denaturing conditions.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/if_selected.jpg
Normal Tissue
Figure 2 Skin
Keratinocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Langerhans Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_B_9_1.jpg
Normal Tissue
Figure 3 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_7_8.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1878/7341_A_5_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000183765-CHEK2
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-CHEK2 specific antibody
- Target
- CHEK2
- Immunogen
- Recombinant human Chk1, aa 1-476.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
- Alternative Names
- Checkpoint Kinase 2; CHK2 Checkpoint Homolog; Cds1 Homolog; HuCds1; RAD53; HCds1; CDS1; CHK2; Serine/Threonine-Protein Kinase Chk2;
- Gene ID
- 11200
- UniProt ID
- O96017
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "CHEK2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4034z | Mouse Anti-CHEK2 Recombinant Antibody (clone 30H3) | WB, ELISA, IF, IHC | Mouse IgG2a, κ |
| MOB-0642MZ | Recombinant Mouse Anti-Human CHEK2 Antibody (clone 9G23) | WB | Mouse antibody |
| VS3-CJ199 | Rabbit Anti-CHEK2 Recombinant Antibody (VS3-CJ199) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| VS3-WK2072 | Rabbit Anti-CHEK2 (pT68) Recombinant Antibody (clone R23-2L-9) | WB, IP | Rabbit IgG |
| VS7-HM707 | Mouse Anti-CHEK2 Recombinant Antibody (clone CBL158HM) | WB, IHC-p, IF, ICC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0122MZ | Chicken Anti-CHEK2 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0689 | Hi-Affi™ Rabbit Anti-CHEK2 Recombinant Antibody (clone DS689AB) | DB, IP, WB | Rabbit IgG |
| MOR-4518 | Hi-Affi™ Rabbit Anti-CHEK2 Recombinant Antibody (clone TH26DS) | WB, IF, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY121 | Mouse Anti-NHP CHEK2 Recombinant Antibody (clone 1C12B8) | WB | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY446 | Anti-CHEK2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1430 | Anti-Mouse CHEK2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1431 | Anti-Canine CHEK2 Immunohistochemistry Kit | IHC |
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