Human Anti-CHIKV E2 Recombinant Antibody (clone ch-CHK-152 N297Q) (CAT#: HPAB-S0185-YC)
A chimeric mouse-human ch-CHK-152 N297Q, an aglycosyl variant, was derived from murine antibody CHK-152, which lacks the ability to engage C1q or Fc-γ receptors; this mutation does not affect the ability to bind the neonatal Fc receptor (FcRn) or half-life of antibody in mouse serum. The affinity of ch-CHK-152 N297Q binding to purified pE2-E1 was measured by surface plasmon resonance (SPR) and compared to the parent murine MAb. Notably, ch-CHK-152 N297Q and the murine CHK-152 all had similar affinity (KD of 3 to 4 nM) and neutralizing activity in cell culture (Fig. 4B). As expected, ch-CHK-152 N297Q failed to bind efficiently to soluble Fc-γ receptors or C1q.
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Figure 1 The effector functions of CHK-152 contribute to protection in vivo.
ch-CHK-152, ch-CHK-152 N297Q, and the murine CHK-152 all had similar affinity and neutralizing activity in cell culture.
Pal, P., Dowd, K. A., Brien, J. D., Edeling, M. A., Gorlatov, S., Johnson, S., ... & Diamond, M. S. (2013). Development of a highly protective combination monoclonal antibody therapy against Chikungunya virus. PLoS pathogens, 9(4), e1003312.
Specifications
- Immunogen
- Adult C57BL/6 mice deficient for interferon regulatory factor 7 (Irf7 −/−) were infected with the La Reunion 2006 OPY-1 strain of CHIKV (CHIKV-LR). These mice were boosted with CHIK virus-like particles, soluble recombinant CHIKV E2 protein, or live CHIKV-LR.
- Host Species
- Human
- Derivation
- Chimeric (mouse/human)
- Type
- Human IgG
- Specificity
- CHIKV E2
- Species Reactivity
- CHIKV
- Clone
- ch-CHK-152 N297Q
- Applications
- Neut, FRNT, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Chikungunya virus E2 protein
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Mouse Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-430 | Recombinant Human Anti-CHIKV E2 Antibody (PABL-430) | IB, ELISA, BL | IgG |
| PABL-431 | Recombinant Mouse Anti-CHIKV E2 Antibody (CHK9) | Neut, BL | IgG |
| MOB-152CQ | Mouse Anti-CHIKV E2 Antibody (CHK265) | Neut, ELISA, IF, FC | Mouse IgG1, λ |
| HPAB-S0184-YC | Human Anti-CHIKV E2 Recombinant Antibody (clone ch-CHK-152) | Neut, FRNT, FuncS | Human IgG |
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