Rabbit Anti-CHRM3 Recombinant Antibody (clone 2D4) (CAT#: ZG-0617J)
This product is a rabbit antibody that recognizes CHRM3.
Specific Inquiry
WB
Figure 1 Rabbit Anti-CHRM3 Antibody (ZG-0617J) in WB
Western Blot
Positive WB detected in:PC3 whole cell lysate
All lanes: Muscarinic Acetylcholine Receptor M3 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kD
IHC
Figure 2 Rabbit Anti-CHRM3 Antibody (ZG-0617J) in IHC
IHC image of ZG-0617J diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human Muscarinic Acetylcholine Receptor M3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CHRM3
- Species Reactivity
- Human
- Clone
- 2D4
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
- Related Disease
- Neuroscience
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- HM3; PBS; EGBRS; muscarinic acetylcholine receptor M3; acetylcholine receptor, muscarinic 3; m3 muscarinic receptor
- Gene ID
- 1131
- UniProt ID
- P20309
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "CHRM3"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1653z | Mouse Anti-CHRM3 Recombinant Antibody (clone 28C2) | IHC | Mouse IgG2b |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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