CytoStream™ Rabbit Anti-CHUK Recombinant Antibody (VS13-YC222) (CAT#: VS13-YC222)
The CytoStream™ Rabbit Anti-CHUK Recombinant Antibody is specific for human and mouse CHUK. CHUK activates NF-kappa-B through kinase activities facilitating proteasomal degradation, regulating inflammation and transcription factors. The anti-CHUK antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1402/60_H6_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1402/60_H6_1_selected.jpg
Figure 2 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1402/4151_A_7_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1402/4151_A_7_8.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1402/4151_B_9_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1402/4151_B_9_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1402/4151_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1402/4151_A_7_3.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1402/4151_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1402/4151_A_8_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1402/4151_A_5_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1402/4151_A_5_6.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000213341-CHUK
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000213341-CHUK
Specifications
- Immunogen
- Recombinant human IKK alpha.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse CHUK
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, IHC-P, FC, IP
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Conserved Helix-Loop-Helix Ubiquitous Kinase; Inhibitor Of Nuclear Factor Kappa-B Kinase Subunit Alpha; Transcription Factor 16; I-Kappa-B Kinase 1; EC 2.7.11.10; IKK-Alpha; NFKBIKA; TCF-16; IKBKA; TCF16; IKK1; IKKA;
- Gene ID
- 1147
- UniProt ID
- O15111
- Long Name
- Conserved Helix-Loop-Helix Ubiquitous Kinase
- Cellular Localization
- Nucleus; Cytoplasm
- Function
- Signal transduction
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IgG Antibody Products
Chicken IgY Antibody Products
Rabbit Monoclonal Antibody Products
Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0883CT | Recombinant Mouse anti-Human CHUK Monoclonal antibody (EML058) | ICC/IF, IHC-P, WB | |
| VS3-QX238 | Mouse Anti-CHUK Recombinant Antibody (clone 3G12H9) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-QX239 | Mouse Anti-CHUK Recombinant Antibody (clone 4C8-C7-E1) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-CJ135 | Rabbit Anti-CHUK Recombinant Antibody (VS3-CJ135) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS13-YC223 | Rabbit Anti-CHUK Recombinant Antibody (VS13-YC223) | WB, ICC, IF, IP | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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