Human Anti-Citrullinated epitope Recombinant Antibody (clone C07) (CAT#: HPAB-AP043-YC)
Autoimmune reactions to certain epitopes of self antigens most likely contribute to the development of rheumatoid arthritis. Often these epitopes are citrullinated. Provided is a novel antibody C07 that can bind to certain citrullinated epitopes namely citrullinated enolase, vimentin, fibrinogen and citrullinated synthetic peptides. The antibody may also be an antibody that has reactivity against only CEP-1, CCP and cit-vim such as antibody C07. The antibody can be used in diagnostics of rheumatoid arthritis, for therapy against rheumatoid arthritis and as research tools.
Specific Inquiry
IHC
Figure 1 Expression of citrullinated targets and peptidylarginine deiminases (PAD) enzymes during different stages of osteoclast (OC) differentiation.
Immunohistochemistry images showing brown 3,3-diaminobenzidene (DAB) staining of citrullinated targets in different stages of differentiation from CD14-positive monocyte precursors to Mφ and mature OCs. Slides were stained with murinised monoclonal anti-citrullinated protein/peptide antibodies (ACPAs) (mB02, mD10, mC07) and a monoclonal control antibody (mE02) and counterstained with haematoxylin (original magnification 500× for CD14-positive monocytes and mature OCs and 250× for the intermediate stages).
Krishnamurthy, A., Joshua, V., Hensvold, A. H., Jin, T., Sun, M., Vivar, N., ... & Magnusson, M. (2016). Identification of a novel chemokine-dependent molecular mechanism underlying rheumatoid arthritis-associated autoantibody-mediated bone loss. Annals of the rheumatic diseases, 75(4), 721-729.
IF
Figure 2 Expression of citrullinated targets and peptidylarginine deiminases (PAD) enzymes during different stages of osteoclast (OC) differentiation.
Confocal microscopy images showing red fluorescence staining with monoclonal murinised ACPAs (mE02, mB02, mD10, mC07) and blue 4′,6-diamidino-2-phenylindole (DAPI) nuclear staining in mature OC.
Krishnamurthy, A., Joshua, V., Hensvold, A. H., Jin, T., Sun, M., Vivar, N., ... & Magnusson, M. (2016). Identification of a novel chemokine-dependent molecular mechanism underlying rheumatoid arthritis-associated autoantibody-mediated bone loss. Annals of the rheumatic diseases, 75(4), 721-729.
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human Citrullinated epitope
- Species Reactivity
- Human
- Clone
- C07
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The Citrullinated epitopes antibody has been reported in applications of Immunohistochemistry, Immunofluorescence, Enzyme-linked Immunosorbent Assay.
For Immunohistochemistry: Cells were fixed with formaldehyde and stained with murinised monoclonal ACPAs (D10, B02, C07), murinised control antibody (E02), rabbit polyclonal anti-PAD2 and monoclonal mouse anti-PADI4 followed by horseradish peroxidase conjugated antimouse antibody as a secondary antibody and 3,3-diaminobenzidene (DAB). The slides were counterstained with Mayer's haematoxylin and viewed using a light microscope.
For Immunofluorescence: The cells were incubated with the murinised ACPAs, polyclonal ACPA and anti-CD68 monoclonal antibody, followed by Alexa-Fluor-633-labeled secondary antibodies (Abcam) and counterstained with 4′,6-diamidino-2-phenylindole.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone C07"
See other products for "Citrullinated epitope"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP042-YC | Human Anti-Citrullinated epitope Recombinant Antibody (clone A40) | ELISA | Human IgG |
| HPAB-AP044-YC | Human Anti-Citrullinated epitope Recombinant Antibody (clone F12) | ELISA | Human IgG |
| HPAB-AP045-YC | Human Anti-Citrullinated epitope Recombinant Antibody (clone 109) | ELISA | Human IgG |
| HPAB-AP046-YC | Human Anti-Citrullinated epitope Recombinant Antibody (clone B07) | ELISA | Human IgG |
| HPAB-AP605-YC | Mouse Anti-Citrullinated epitope Recombinant Antibody (clone 12G1) | ELISA, IHC, WB | Mouse IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP042-YC-S(P) | Human Anti-Citrullinated epitope Recombinant Antibody (clone A40); scFv Fragment | ELISA | Human scFv |
| HPAB-AP043-YC-S(P) | Human Anti-Citrullinated epitope Recombinant Antibody (clone C07); scFv Fragment | ELISA | Human scFv |
| HPAB-AP044-YC-S(P) | Human Anti-Citrullinated epitope Recombinant Antibody (clone F12); scFv Fragment | ELISA | Human scFv |
| HPAB-AP045-YC-S(P) | Human Anti-Citrullinated epitope Recombinant Antibody (clone 109); scFv Fragment | ELISA | Human scFv |
| HPAB-AP046-YC-S(P) | Human Anti-Citrullinated epitope Recombinant Antibody (clone B07); scFv Fragment | ELISA | Human scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP046-YC-F(E) | Human Anti-Citrullinated epitope Recombinant Antibody (clone B07); Fab Fragment | ELISA | Human Fab |
| HPAB-AP047-YC-F(E) | Human Anti-Citrullinated epitope Recombinant Antibody (clone A03); Fab Fragment | ELISA | Human Fab |
| HPAB-AP048-YC-F(E) | Human Anti-Citrullinated epitope Recombinant Antibody (clone B05); Fab Fragment | ELISA | Human Fab |
| HPAB-AP049-YC-F(E) | Human Anti-Citrullinated epitope Recombinant Antibody (clone C05); Fab Fragment | ELISA | Human Fab |
| HPAB-AP050-YC-F(E) | Human Anti-Citrullinated epitope Recombinant Antibody (clone C04); Fab Fragment | ELISA | Human Fab |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for HPAB-AP043-YC. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.