Mouse Anti-Citrullinated epitope Recombinant Antibody (HPAB-AP605-YC) (CAT#: HPAB-AP605-YC)
A monoclonal antibody (HPAB-AP605-YC) which specifically detects proteins with a citrulline residue is provided to detect citrullinated proteins and to diagnose and treatment studies of autoimmune diseases. The antibody can detect the citrulline screen peptide present in the tissue of patients with RA specifically it was confirmed by immunohistochemical staining.
Specific Inquiry
SDS-PAGE
Figure 1 Mouse Anti-Citrullinated epitope Recombinant Antibody (HPAB-AP605-YC) in SDS-PAGE
SDS-PAGE analysis of HPAB-AP605-YC in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 180-230 kDa.
SEC-HPLC
Figure 2 Mouse Anti-Citrullinated epitope Recombinant Antibody (HPAB-AP605-YC) in HPLC
The purity of HPAB-AP605-YC was greater than 95% as determined by SEC-HPLC.
WB
Figure 3 Mouse Anti-Citrullinated epitope Recombinant Antibody-Biotin (HPAB-AP605-YC) in WB
Lane 1: HPAB-AP605-YC in non-reducing condition (500 ng)
Lane 2: HPAB-AP605-YC-Biotin in non-reducing condition (500 ng)
Detection agent: SA-HRP
WB
Figure 4 Mouse Anti-Citrullinated epitope Recombinant Antibody (clone 12G1), mouse IgG2a (HPAB-AP605-YC-mG2a) in WB
Western blot analysis of HPAB-AP605-YC-mG2a was performed by loading CCP peptide (HQCHQEST-{{Cit}}-GRSRGRCGRSGS(Disulfide bridge: Cys3-Cys16)).
HPAB-AP605-YC-mG2a incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat anti-Mouse IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
ELISA
Figure 5 Mouse Anti-Citrullinated epitope Recombinant Antibody (clone 12G1), mouse IgG2a (HPAB-AP605-YC-mG2a) in ELISA
ELISA analysis of HPAB-AP605-YC-mG2a was performed by coating with CCP peptide (HQCHQEST-{{Cit}}-GRSRGRCGRSGS(Disulfide bridge: Cys3-Cys16)).
The secondary antibody: HRP-Goat anti-Mouse IgG
DB
Figure 6 Mouse Anti-Citrullinated epitope Recombinant Antibody (clone 12G1), mouse IgG2a (HPAB-AP605-YC-mG2a) in DB
Dot Blot analysis of HPAB-AP605-YC-mG2a was performed by coating with CCP peptide (HQCHQEST-{{Cit}}-GRSRGRCGRSGS(Disulfide bridge: Cys3-Cys16)).
The secondary antibody: HRP-Goat anti-Mouse IgG
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human Citrullinated epitope
- Species Reactivity
- Human
- Applications
- ELISA, IHC, WB
- Related Disease
- Rheumatoid arthritis
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The Citrullinated Protein antibody has been reported in applications of Immunohistochemistry, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Western Blot.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
ScFv Antibody Products
Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP042-YC | Human Anti-Citrullinated epitope Recombinant Antibody (clone A40) | ELISA | Human IgG |
| HPAB-AP043-YC | Human Anti-Citrullinated epitope Recombinant Antibody (clone C07) | ELISA | Human IgG |
| HPAB-AP044-YC | Human Anti-Citrullinated epitope Recombinant Antibody (clone F12) | ELISA | Human IgG |
| HPAB-AP045-YC | Human Anti-Citrullinated epitope Recombinant Antibody (clone 109) | ELISA | Human IgG |
| HPAB-AP046-YC | Human Anti-Citrullinated epitope Recombinant Antibody (clone B07) | ELISA | Human IgG |
Customer Reviews and Q&As
Customer Review
Q&As
-
Adaptable with Varying Detergents
Even in the presence of light detergents employed in research on membrane proteins, it demonstrated steady function. -
Problems with Zero Aggregation
Not even after extended storage was there any aggregation. Assures dependability and regular outcomes. -
Sophisticated Validation Support
The supplier's recommended validation procedures were comprehensive and efficient, which made integration into new assays simple.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-AP605-YC, RRID: AB_3111430)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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