Mouse Anti-Cleaved PARP Recombinant Antibody (ZG-0166J) (CAT#: ZG-0166J)
This product is a mouse antibody that recognizes Cleaved PARP.
Specific Inquiry
IF
Figure 1 Mouse Anti-Cleaved PARP Recombinant Antibody (ZG-0166J) in IF
Immunofluorescence analysis of Hela cell. 1.BRCA1 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min).
IF
Figure 2 Mouse Anti-Cleaved PARP Recombinant Antibody (ZG-0166J) in IF
Immunofluorescence analysis of human-liver-cancer tissue. 1.Cleaved PARP Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary Antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target.Picture B: DAPI. Picture C: merge of A+B
IHC
Figure 3 Mouse Anti-Cleaved PARP Recombinant Antibody (ZG-0166J) in IHC
Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1.Cleaved PARP Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.
IHC
Figure 4 Mouse Anti-Cleaved PARP Recombinant Antibody (ZG-0166J) in IHC
Western blot analysis of Jurkat, diluted at 1:3000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.
Specifications
- Immunogen
- Synthetic Peptide of Cleaved PARP
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human Cleaved PARP
- Species Reactivity
- Human
- Applications
- IF/ICC, WB, IHC-P
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunofluorescence/Immunocytochemistry, Western Blot, Immunohistochemistry-Paraffin.
Target
- Alternative Names
- PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1; poly [ADP-ribose] polymerase 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase NAD(+); ADP-ribosyltransferase diphtheria toxin-like 1; DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1; poly (ADP-ribose) polymerase family, member 1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; poly[ADP-ribose] synthase 1; protein poly-ADP-ribosyltransferase PARP1
- Gene ID
- 142
- UniProt ID
- P09874
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0165J | Mouse Anti-Cleaved PARP Recombinant Antibody (clone M4) | WB | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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