Mouse Anti-Cleaved PARP Recombinant Antibody (ZG-0166J) (CAT#: ZG-0166J)
This product is a mouse antibody that recognizes Cleaved PARP.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
IF
Figure 1 Mouse Anti-Cleaved PARP Recombinant Antibody (ZG-0166J) in IF
Immunofluorescence analysis of Hela cell. 1.BRCA1 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min).
IF
Figure 2 Mouse Anti-Cleaved PARP Recombinant Antibody (ZG-0166J) in IF
Immunofluorescence analysis of human-liver-cancer tissue. 1.Cleaved PARP Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary Antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target.Picture B: DAPI. Picture C: merge of A+B
IHC
Figure 3 Mouse Anti-Cleaved PARP Recombinant Antibody (ZG-0166J) in IHC
Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1.Cleaved PARP Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.
IHC
Figure 4 Mouse Anti-Cleaved PARP Recombinant Antibody (ZG-0166J) in IHC
Western blot analysis of Jurkat, diluted at 1:3000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.
Specifications
- Immunogen
- Synthetic Peptide of Cleaved PARP
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human Cleaved PARP
- Species Reactivity
- Human
- Applications
- IF, ICC, WB, IHC-P
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunofluorescence/Immunocytochemistry, Western Blot, Immunohistochemistry-Paraffin.
Target
- Alternative Names
- PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1; poly [ADP-ribose] polymerase 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase NAD(+); ADP-ribosyltransferase diphtheria toxin-like 1; DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1; poly (ADP-ribose) polymerase family, member 1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; poly[ADP-ribose] synthase 1; protein poly-ADP-ribosyltransferase PARP1
- Gene ID
- 142
- UniProt ID
- P09874
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0165J | Mouse Anti-Cleaved PARP Recombinant Antibody (clone M4) | WB | Mouse IgG |
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