Rabbit Anti-CLIC1 Recombinant Antibody (clone R08-4A2)
CAT#: VS3-FY1987
This is a rabbit recombinant monoclonal antibody that detects human CLIC1 protein, a calculated weight of 27 kDa. It cross-reacts with mouse, rat, hamster. It is suitable for use in a variety of assays, including WB, ICC/IF, IP.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40557/if_selected.jpg
Normal Tissue
Figure 2 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20825/120231_A_8_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20825/120231_A_9_3.jpg
Normal Tissue
Figure 4 Gallbladder
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/20825/120231_A_5_4.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000213719-CLIC1
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WB
Figure 1 Anti-CLIC1 Antibody (VS3-FY1987) in WB.
The CLIC1 expression in Raw264.7, mouse kidney, mouse spleen lysates were detected using the anti-CLIC1 by western blot assay.
WB
Figure 2 Anti-CLIC1 Antibody (VS3-FY1987) in WB.
The CLIC1 expression in Hela, CHO-K1, C6, Ramos lysates were detected using the anti-CLIC1 by western blot assay.
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Specifications
- Immunogen
- Recombinant human CLIC1 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat, Hamster CLIC1
- Species Reactivity
- Human, Mouse, Rat, Hamster
- Clone
- R08-4A2
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 27 kDa, while the observed molecular weight stands at 27 kDa.
- Related Disease
- Intrahepatic Gall Duct Cancer and Gallbladder Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Chloride Intracellular Channel 1; Regulatory Nuclear Chloride Ion Channel Protein; Nuclear Chloride Ion Channel 27; Chloride Channel ABP; NCC27; HRNCC; G6
- Gene ID
- 1192
- UniProt ID
- O00299
- Sequence Similarities
- Belongs to the chloride channel CLIC family.
- Cellular Localization
- Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus
- Post Translation Modifications
- Hydrogen peroxide treatment causes a conformation change, leading to dimerization and formation of an intramolecular disulfide bond between Cys-24 and Cys-59. (O00299-CLIC1_HUMAN)
Ubiquitination at Lys49, Lys57, Lys119, Lys131, Lys135, Lys138, Lys166, and Lys183 (NX_O00299 [NX_O00299-1])
Modification sites at PhosphoSitePlus (O00299)
Glycosylation from GlyGen (O00299) 2 sites, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001274522.1; NP_001274523.1; NP_001279.2
- Function
- Can insert into membranes and form chloride ion channels. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Involved in regulation of the cell cycle.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CLIC1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3786z | Mouse Anti-CLIC1 Recombinant Antibody (clone 18A8) | WB, ELISA, ICC, IF, IHC | Mouse IgG1, κ |
| MOB-1113MZ | Recombinant Mouse Anti-Human CLIC1 Antibody | WB | Mouse antibody |
| VS-0923-FY40 | Recombinant Mouse Anti-CLIC1 Antibody (VS-0923-FY40) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY468 | Anti-CLIC1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1510 | Anti-Human CLIC1 Immunohistochemistry Kit | IHC |
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