This recombinant monoclonal antibody to Clostridium botulinum HA-33 is a Mouse monoclonal antibody that can be used for applications: ELISA. This antibody is a thermostabilized antibody.
Figure 1 ELISA signal before and after the thermal challenges.
(b) ELISA signal before and after the thermal challenges. Wildtype and each variant underwent thermal challenge of heating at 70 °C for 1 hour or 2 hours before OD450 was measured. (c) Summary of binding affinities (EC50) to HA33 before and after thermal challenges of 1 hour or 2 hours.
Lee, J., Der, B. S., Karamitros, C. S., Li, W., Marshall, N. M., Lungu, O. I., ... & Ellington, A. D. (2020). Computer‐based engineering of thermostabilized antibody fragments. AIChE Journal, 66(3), e16864.
Figure 2 ELISA curves for the wildtype, C1+C9 and C9+C14 scFv before and after thermal challenge.
All the thermal challenge experiments (70 °C) were performed with 1 mg/mL of protein. Data are from in triplicate experiments with error indicating s.d.
Lee, J., Der, B. S., Karamitros, C. S., Li, W., Marshall, N. M., Lungu, O. I., ... & Ellington, A. D. (2020). Computer‐based engineering of thermostabilized antibody fragments. AIChE Journal, 66(3), e16864.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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