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    Rabbit Anti-CLP1 Recombinant Antibody (clone R53-4N-9) (CAT#: VS3-FY2161)

    We offer this specifically rabbit recombinant monoclonal antibody, which was purified by affinity chromatography. It detects human CLP1 in WB, IHC-P, FC assay. The R53-4N-9 is recommended for dilution with western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/100-1/200), flow cytometry (dilution 1/50).

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    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
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    Conjugation :
    Fc Engineering :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/57770/1116_B10_1_selected.jpg
    Normal Tissue
    Figure 2 Cerebral cortex
    Figure 2 Cerebral cortex
    (Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/57770/130500_B_9_5.jpg
    Figure 3 Colon
    Figure 3 Colon
    (Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/57770/130500_A_8_3.jpg
    Figure 4 Liver
    Figure 4 Liver
    (Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/57770/130500_A_9_4.jpg
    Figure 5 Kidney
    Figure 5 Kidney
    (Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/57770/130500_A_7_5.jpg
    Figure 6 Testis
    Figure 6 Testis
    (Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/57770/130500_A_5_6.jpg
    Figure 7 Bone marrow
    Figure 7 Bone marrow
    (Hematopoietic cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/57770/130500_B_6_4.jpg
    Figure 8 Lymph node
    Figure 8 Lymph node
    (Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/57770/130500_A_8_8.jpg
    RNA Expression
    Figure 9 RNA cell line category: Low cell line specificity
    Figure 9 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172409-CLP1

    Specifications

    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human CLP1
    • Species Reactivity
    • Human
    • Clone
    • R53-4N-9
    • Applications
    • Western Blot, Immunohistochemistry-Paraffin, Flow Cytometry
    • Conjugate
    • Unconjugated
    • MW
    • The calculated weight of the target protein is approximately 48 kDa, while the observed molecular weight stands at 48kDa.
    • Related Disease
    • Pontocerebellar Hypoplasia, Type 10 and Pontocerebellar Hypoplasia

    Product Property

    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS (pH 7.4), 150 mM NaCl, 50% glycerol
    • Preservative
    • 0.02% Sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • Western Blot: 1/500-1/1000
      Immunohistochemistry: 1/100-1/200
      Flow Cytometry: 1/50

    Target

    • Alternative Names
    • Cleavage And Polyadenylation Factor I Subunit 1; Pre-MRNA Cleavage Complex II Protein Clp1; Polyadenylation Factor Clp1; Polynucleotide Kinase Clp1; EC 2.7.1.78; HEAB; CLP1, Cleavage And Polyadenylation Factor I Subunit, Homolog (S. Cerevisiae)
    • Sequence Similarities
    • Belongs to the Clp1 family. Clp1 subfamily.
    • Cellular Localization
    • Nucleus
    • Post Translation Modifications
    • Ubiquitination at Lys127 (NX_Q92989 [NX_Q92989-1])
      Modification sites at PhosphoSitePlus (Q92989)
    • Function
    • Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA.

    Recommended Products

    Isotype Control

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "CLP1"

    Select a product category from the dropdown menu below to view related products.
    Please select product type
    IgG Antibody Products
    CAT Product Name Application Type
    MOB-0150CT Recombinant Mouse anti-Human CLP1 Monoclonal antibody (EML1217) WB

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