Mouse Anti-COL1A1 Recombinant Antibody (clone 4E8G11) (CAT#: VS7-HM757)
Mouse Anti-COL1A1 Recombinant Antibody (clone 4E8G11) is an mouse IgG1 antibody that recognizes the COL1A1 by FCM.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12111/1934_F8_3_cr5d1ca7aa8d4b0_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12111/1934_F8_3_cr5d1ca7aa8d4b0_selected.jpg
Figure 2 IHC staining of human colon
(Immunohistochemical staining of human colon shows extracellular positivity.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/ihc_selected.jpg
(Immunohistochemical staining of human colon shows extracellular positivity.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/ihc_selected.jpg
Figure 3 IF staining of human cell line BJ
(Immunofluorescent staining of human cell line BJ shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12111/1936_A7_4_blue_red_green.jpg
(Immunofluorescent staining of human cell line BJ shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12111/1936_A7_4_blue_red_green.jpg
Figure 4 Cerebral cortex
(Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/32976_B_7_5.jpg
(Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/32976_B_7_5.jpg
Figure 5 Kidney
(Cells in tubules
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/32976_A_8_5.jpg
(Cells in tubules
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/32976_A_8_5.jpg
Figure 6 Testis
(Leydig cells
Staining:High
Intensity: Strong
Quantity: 75%-25% Preleptotene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/32976_A_5_6.jpg
(Leydig cells
Staining:High
Intensity: Strong
Quantity: 75%-25% Preleptotene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/32976_A_5_6.jpg
Figure 7 Skin
(Fibroblasts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Keratinocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/32976_B_9_1.jpg
(Fibroblasts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Keratinocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11795/32976_B_9_1.jpg
Figure 8 RNA cell line category: Cell line enhanced (ASC TERT1, BJ, fHDF/TERT166, HSkMC, U-138 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000108821-COL1A1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000108821-COL1A1
Specifications
- Immunogen
- Purified recombinant human COL1A1 (AA: 1219-1464) fragment expressed in E. Coli.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1
- Specificity
- COL1A1
- Species Reactivity
- Human
- Clone
- 4E8G11
- Applications
- FCM
- Conjugate
- None
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity-chromatography
- Format
- Liquid
- Molecular Weight
- 139kDa
- Concentration
- Please refer to the tube label.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide.
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice pack
Target
- Alternative Names
- COL1A1; collagen, type I, alpha 1; collagen alpha-1(I) chain; OI4; alpha-1 type I collagen; pro-alpha-1 collagen type 1; collagen alpha 1 chain type I; collagen alpha-1(I) chain preproprotein; collagen of skin, tendon and bone, alpha-1 chain;
Recommended Products
Isotype Control
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-706-VHH | Recombinant Anti-Human COL1A1 VHH Single Domain Antibody | IHC, IP, FC, Neut, FUNC | Llama VHH |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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