Mouse Anti-COL2A1 Recombinant Antibody (clone 7F9) (CAT#: VS3-CJ279)

• Immunogen
• Synthetic peptide of Collagen II

• Host Species
• Mouse

• Specificity
• Human, Mouse and Rat COL2A1

• Species Reactivity
• Human, Mouse, Rat

• Clone
• 7F9

• Applications
• IHC-P

• Conjugate
• Unconjugated

• Purification
• Immunogen affinity chromatography

• Purity
• >95% as determined by SDS-PAGE

• Concentration
• 1 mg/mL (lot specific)

• Buffer
• 50% Glycerol, PBS, pH7.4.

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Immunohistochemistry-Parraffin (1:50-1:300).

• Alternative Names
• AOM; ANFH; SEDC; STL1; COL11A3; Collagen alpha-1(II) chain; Alpha-1 type II collagen

• Gene ID
• 1280

• UniProt ID
• P02458

• Sequence Similarities
• Belongs to the fibrillar collagen family.

• Cellular Localization
• Extracellular matrix, Secreted

• Post Translation Modifications
• The N-telopeptide is covalently linked to the helical COL2 region of alpha 1(IX), alpha 2(IX) and alpha 3(IX) chain. The C-telopeptide is covalently linked to an another site in the helical region of alpha 3(IX) COL2.
  Contains mostly 4-hydroxyproline. Prolines at the third position of the tripeptide repeating unit (G-X-P) are 4-hydroxylated in some or all of the chains.
  Contains 3-hydroxyproline at a few sites. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline.
  Lysine residues at the third position of the tripeptide repeating unit (G-X-Y) are 5-hydroxylated in some or all of the chains.
  O-glycosylated on hydroxylated lysine residues. The O-linked glycan consists of a Glc-Gal disaccharide.

• Protein Refseq
• NP_001835.3; NP_149162.2

• Function
• Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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