Mouse Anti-COL2A1 Recombinant Antibody (clone ACC1) (CAT#: PABC-248)

Figure 1 ELISA

Binding of ACC1 for cyclic C1-CIT360, C1-CIT365, C1-R360 and C1-R365 were measured by ELISA.

Ge, C., Tong, D., Liang, B., Lönnblom, E., Schneider, N., Hagert, C., ... & Fields, G. B. (2017). Anti-citrullinated protein antibodies cause arthritis by cross-reactivity to joint cartilage. JCI insight, 2(13).

Figure 2 Characterization of ACC1 antibody

Reactivity of ACC1 towards denatured CI, CII, CIX, and CXI in ELISA. The Corning Costar plates (Thermo Fisher Scientific) were coated for 2 h with 5 μg/ml denatured collagen (70 °C, 30 min) and blocked with 3% heat-inactivated FBS in DPBS for 2 h at room temperature. Specific binding was detected using HRP-conjugated anti-mouse immunoglobulin kappa light chain antibody (clone 187.1; Southern Biotech) and ABTS (Roche) as substrate. Absorbance at 405 nm was measured with a Synergy-2 (BioTek Instruments).

Ge, C., Tong, D., Liang, B., Lönnblom, E., Schneider, N., Hagert, C., ... & Fields, G. B. (2017). Anti-citrullinated protein antibodies cause arthritis by cross-reactivity to joint cartilage. JCI insight, 2(13).

Figure 3 Inhibition

Solid-phase inhibition assay. The ELISA plate coated by C1-CIT360 or C1-CIT365, was pre-incubated with ACC1 (2ug/ml) and then the sera (1:300 dilution) of a pool of 6 RA patients selected for positivity against C1-CIT360 was added for 3 hours of incubation at RT. The binding of human polyclonal antibodies to the given peptides was measured. One representative experiment was shown from three assays performed using triplicate technical replicates. Data represent mean + SD and the p value was calculated by Mann-Whitney U test to compare different groups.

Ge, C., Tong, D., Liang, B., Lönnblom, E., Schneider, N., Hagert, C., ... & Fields, G. B. (2017). Anti-citrullinated protein antibodies cause arthritis by cross-reactivity to joint cartilage. JCI insight, 2(13).

Figure 4 Immunohistochemical staining

The cartilage binding of ACC1 to mouse cartilage was evaluated by immunohistochemical staining in vitro.

Ge, C., Tong, D., Liang, B., Lönnblom, E., Schneider, N., Hagert, C., ... & Fields, G. B. (2017). Anti-citrullinated protein antibodies cause arthritis by cross-reactivity to joint cartilage. JCI insight, 2(13).