Mouse Anti-COX4I1 Recombinant Antibody (clone 6C8) (CAT#: ZG-0188J)
This product is a mouse antibody that recognizes COX4I1.
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IF
Figure 1 Mouse Anti-COX4I1 Recombinant Antibody (clone 6C8) in IF
Immunofluorescence analysis of Hela cell. 1, AF-10 Polyclonal Antibody(red) was diluted at 1:200 (4°C overnight). COX IV Monoclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
IF
Figure 2 Mouse Anti-COX4I1 Recombinant Antibody (clone 6C8) in IF
Immunofluorescence analysis of Mouse-kidney tissue. 1.COX IV Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary Antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI.Picture C: merge of A+B
IHC
Figure 3 Mouse Anti-COX4I1 Recombinant Antibody (clone 6C8) in IHC
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1.COX IV Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.
IHC
Figure 4 Mouse Anti-COX4I1 Recombinant Antibody (clone 6C8) in IHC
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.COX IV Monoclonal Antibody was diluted at 1:200 (4°C, overnight).2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min).3, Secondary Antibody was diluted at 1:200 (room temperature, 30min).Negative control was used by secondary Antibody only.
WB
Figure 5 Mouse Anti-COX4I1 Recombinant Antibody (clone 6C8) in WB
Western blot analysis of lysates from 1) COS7, 2) 3T3, 3) Hela cells, (Green) primary Antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary Antibody was diluted at 1:10000, 37°C 1 hour. (Red) Actin β Polyclonal Antibody Antibody was diluted at 1:5000 as loading control, 4°Cover night, Dylight 680 secondary Antibody was diluted at 1:10000, 37°C1 hour.
WB
Figure 6 Mouse Anti-COX4I1 Recombinant Antibody (clone 6C8) in WB
Western blot analysis of Hela, diluted at 1) 1:2000 2) 1:5000
Specifications
- Immunogen
- Recombinant Protein of Cytochrome c oxidase subunit 4 isoform 1,
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human COX4I1
- Species Reactivity
- Human, Rat, Mouse
- Clone
- 6C8
- Applications
- WB, IHC-P, IF/ICC
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence/Immunocytochemistry.
Target
- Alternative Names
- COX4; COXIV; COX4-1; COXIV-1; MC4DN16; COX IV-1; cytochrome c oxidase subunit 4 isoform 1, mitochondrial; cytochrome c oxidase polypeptide IV; cytochrome c oxidase subunit IV
- Gene ID
- 1327
- UniProt ID
- P13073
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0768 | Hi-Affi™ Rabbit Anti-COX4I1 Recombinant Antibody (clone DS768AB) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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