Mouse Anti-COX4I1 Recombinant Antibody (clone 6C8) (CAT#: ZG-0188J)

Figure 1 Mouse Anti-COX4I1 Recombinant Antibody (clone 6C8) in IF

Immunofluorescence analysis of Hela cell. 1, AF-10 Polyclonal Antibody(red) was diluted at 1:200 (4°C overnight). COX IV Monoclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Figure 2 Mouse Anti-COX4I1 Recombinant Antibody (clone 6C8) in IF

Immunofluorescence analysis of Mouse-kidney tissue. 1.COX IV Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary Antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI.Picture C: merge of A+B

Figure 3 Mouse Anti-COX4I1 Recombinant Antibody (clone 6C8) in IHC

Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1.COX IV Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.

Figure 4 Mouse Anti-COX4I1 Recombinant Antibody (clone 6C8) in IHC

Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.COX IV Monoclonal Antibody was diluted at 1:200 (4°C, overnight).2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min).3, Secondary Antibody was diluted at 1:200 (room temperature, 30min).Negative control was used by secondary Antibody only.

Figure 5 Mouse Anti-COX4I1 Recombinant Antibody (clone 6C8) in WB

Western blot analysis of lysates from 1) COS7, 2) 3T3, 3) Hela cells, (Green) primary Antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary Antibody was diluted at 1:10000, 37°C 1 hour. (Red) Actin β Polyclonal Antibody Antibody was diluted at 1:5000 as loading control, 4°Cover night, Dylight 680 secondary Antibody was diluted at 1:10000, 37°C1 hour.

Figure 6 Mouse Anti-COX4I1 Recombinant Antibody (clone 6C8) in WB

Western blot analysis of Hela, diluted at 1) 1:2000 2) 1:5000