Mouse Anti-COX4I1 Recombinant Antibody (VS3-CJ625) (CAT#: VS3-CJ625)

• Immunogen
• Recombinant protein corresponding to human COX4-1

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human, Mouse, Rat endogenous levels of COX4-1 protein

• Species Reactivity
• Human, Mouse, Rat

• Applications
• WB, ICC, IF

• Conjugate
• Unconjugated

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 30% Glycerol, 0.87% NaCl, 0.42% Potassium phosphate, pH7.3.

• Preservative
• 0.01% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Shipped at 4°C

• Application Notes
• This antibody has been tested for use in Western Blot (1:1000-1:3000), Immunocytochemistry/Immunofluorescence (1:100-1:200).

• Alternative Names
• COX4; COXIV; COX4-1; COXIV-1; MC4DN16; COX IV-1

• Gene ID
• 1327

• UniProt ID
• P13073

• Sequence Similarities
• Belongs to the cytochrome c oxidase IV family.

• Cellular Localization
• Membrane, Mitochondrion, Mitochondrion inner membrane

• Protein Refseq
• NP_001305715.1; NP_001305717.1; NP_001305723.1

• Function
• Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

• 2ND-042CQ
• Rabbit Anti-Mouse IgG (H&L) Antibody

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