Mouse Anti-Cr2 Recombinant Antibody (clone 4B2) (CAT#: FAMAB-0109JF)

Recombinant mouse monoclonal antibody binds to CR2/CR1. This anti-CR2/CR1 antibody (4B2) can be as a new tool to study the effects of chronic complement receptor inhibition in murine models of human diseases.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
ELISA

Figure 1 Effect of HB5 anti-CR2 MAb on the in vifro response of adult (squares) and neonatal (circles) B cells to PS4.

Figure 1 Effect of HB5 anti-CR2 MAb on the in vifro response of adult (squares) and neonatal (circles) B cells to PS4.

B cells were activated with PS4 (closed symbols) in tissue culture tubes precoated with varying concentrations of HB5.

Griffioen, A. W., Toebes, E. A., Zegers, B. J., & Rijkers, G. T. (1992). Role of CR2 in the human adult and neonatal in vitro antibody response to type 4 pneumococcal polysaccharide. Cellular immunology, 143(1), 11-22.

FC

Figure 2 Spleen cells were serially incubated on ice with 4B2 scFv.

Figure 2 Spleen cells were serially incubated on ice with 4B2 scFv.

Cells were then washed twice in PBS–1% BSA and analysed. Cells with forward and sideward scatter characteristics of lymphocytes were gated.

Prechl, J., Tchorbanov, A., Horváth, A., Baiu, D. C., Hazenbos, W., Rajnavölgyi, É., ... & Erdei, A. (1999). Targeting of influenza epitopes to murine CR1/CR2 using single-chain antibodies. Immunopharmacology, 42(1-3), 159-165.

WB

Figure 3 Samples were denatured, run on 12% SDS-acrylamide gel, blotted on nitrocellulose and then detected by Abs recognizing either the N terminal IP epitope.

Figure 3 Samples were denatured, run on 12% SDS-acrylamide gel, blotted on nitrocellulose and then detected by Abs recognizing either the N terminal IP epitope.

Markers show molecular weight in kDas.

Prechl, J., Tchorbanov, A., Horváth, A., Baiu, D. C., Hazenbos, W., Rajnavölgyi, É., ... & Erdei, A. (1999). Targeting of influenza epitopes to murine CR1/CR2 using single-chain antibodies. Immunopharmacology, 42(1-3), 159-165.


Specifications

  • Immunogen
  • Recombinant mouse CR2 protein
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1, κ
  • Specificity
  • Mouse CR2/CR1
  • Species Reactivity
  • Mouse
  • Clone
  • 4B2
  • Applications
  • WB, ELISA, FC
  • Related Disease
  • Autoimmune disease

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The Cr2 antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Flow Cytometry.

Target

  • Alternative Names
  • Cr1; C3DR; CD21; CD35; Cr-1; Cr-2
  • Function
  • Blocking

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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Neutralizing Antibody

CAT Product Name Application Type
NEUT-497CQ Rabbit Anti-Cr2 Recombinant Antibody (clone 7G6) WB, Neut, FC, IHC-Fr, IP, CyTOF® Rabbit IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0795 Hi-Affi™ Rabbit Anti-CR2 Recombinant Antibody (clone DS795AB) ELISA Rabbit IgG

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC3372 PE-B*15:16/Human CR2 (VSYGSIVTY) MHC Tetramer FCM

Fab Fragment Antibody

CAT Product Name Application Type
FAMAB-0109JF-F(E) Mouse Anti-Cr2 Recombinant Antibody (clone 4B2); Fab Fragment FC, WB, ELISA, FuncS Mouse Fab

scFv Fragment Antibody

CAT Product Name Application Type
FAMAB-0109JF-S(P) Mouse Anti-Cr2 Recombinant Antibody (clone 4B2); scFv Fragment FC, WB, ELISA, FuncS Mouse scFv

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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