Mouse Anti-CREB1 Recombinant Antibody (ZG-0435F)
CAT#: ZG-0435F
This product is a mouse antibody that recognizes CREB1.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-CREB1 Recombinant Antibody (ZG-0435F) in WB.
Using CREB-1 monoclonal antibodies against K562 (1), Jurkat (2), L1210 (3), HEK293 (4), A431 (5), HeLa (6), Cos7 (7), PC-12 (8) Perform Western blot analysis) and NIH/3T3 (9) cell lysate.
IHC
Figure 2 Mouse Anti-CREB1 Recombinant Antibody (ZG-0435F) in IHC.
The CREB-1 monoclonal antibody was used for immunohistochemical analysis of paraffin-embedded prostate cancer tissue (left) and submandibular tumor tissue (right) by DAB staining.
IF
Figure 3 Mouse Anti-CREB1 Recombinant Antibody (ZG-0435F) in IF.
Immunofluorescence analysis of Hela cells using CREB-1 monoclonal antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
ELISA
Figure 4 Mouse Anti-CREB1 Recombinant Antibody (ZG-0435F) in ELISA.
ELISA analysis of ZG-0435F was performed by coating with human CREB1 Protein.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19150/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3803/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19150/ihc_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19150/44743_A_7_8.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19150/44743_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19150/44743_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19150/44743_A_7_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19150/44743_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19150/44743_A_6_6.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118260-CREB1
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Specifications
- Immunogen
- Recombinant fragment of human CREB-1 expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CREB1
- Species Reactivity
- Human, Mouse, Rat, Monkey
- Applications
- Western Blot, Immunohistochemistry, Immunofluorescence, Enzyme-linked Immunosorbent Assay
- Conjugate
- NONE
Product Property
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/200-1/1000.
Immunofluorescence: 1/200-1/1000
ELISA: 1/10000
Target
- Alternative Names
- CAMP Responsive Element Binding Protein 1; CREB-1; Cyclic Adenosine 3,5-Monophosphate Response Element-Binding Protein CREB; Cyclic AMP-Responsive Element-Binding Protein 1; CAMP-Responsive Element-Binding Protein 1; CAMP-Response Element-Binding Protein-1; Active Transcription Factor CREB; Transactivator Protein; CREB; CREB1; Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1
- Gene ID
- 1385
- UniProt ID
- P16220
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Related Signaling Pathways
cAMP Signaling Pathway
MAPK Signaling Pathway
PPAR Signaling Pathway
TLR Signaling Pathway
TNF Signaling Pathway
Related Diseases
Prostate Cancer
Morphine Addiction
Cocaine Addiction
Amphetamine Addiction
Alcoholism
Cushing Syndrome
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "CREB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1803z | Mouse Anti-CREB1 Recombinant Antibody (clone 30G3) | WB, ELISA, ICC, IF, IHC | Mouse IgG1 |
| MOB-0399CT | Recombinant Mouse anti-Human CREB1 Monoclonal antibody (93C625) | WB | |
| MRO-1788-CN | Rabbit Anti-CREB1 Polyclonal Antibody (MRO-1788-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0382U | Rabbit Anti-CREB1 Recombinant Antibody (clone Q19-D) | ELISA, WB | Rabbit IgG |
| ZG-0432U | Rabbit Anti-CREB1 Recombinant Antibody (clone A17-A), FITC | FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0781 | Hi-Affi™ Rabbit Anti-CREB1 Recombinant Antibody (clone DS781AB) | WB, IP | Rabbit IgG |
| MOR-0800 | Hi-Affi™ Rabbit Anti-CREB1 Recombinant Antibody (clone DS800AB) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
| MOR-4560 | Hi-Affi™ Rabbit Anti-CREB1 Recombinant Antibody (clone TH70DS) | WB, IF, ICC, FC, ChIP | Rabbit IgG |
| MOR-4561 | Hi-Affi™ Rabbit Anti-CREB1 Recombinant Antibody (clone TH71DS) | WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY69 | AbPlus™ Anti-CREB1 Magnetic Beads (CBACN-159) | IP, Protein Purification | |
| VS-0724-YC812 | AbPlus™ Anti-CREB1 Magnetic Beads (VS-0724-YC812) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY538 | Anti-CREB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1663 | Anti-Mouse CREB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1662 | Anti-Human CREB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1664 | Anti-Rat CREB1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC244 | CytoStream™ Rabbit Anti-CREB1 Recombinant Antibody (VS13-YC244) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
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