Mouse Anti-CST3 Recombinant Antibody (clone 4A9-8D8-1D5)
CAT#: VS3-QX288
This product is a mouse antibody that recognizes CST3. The antibody can be used in applications: WB, IHC, ELISA.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13143/97_A12_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/118/if_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to the Cytosol
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/118/642_E9_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/118/2084_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/118/2084_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/118/2084_A_7_4.jpg
Normal Tissue
Figure 7 Kidney
Proximal tubules (cell body)
Staining:High
Intensity: Strong
Quantity: 75%-25%
Proximal tubules (microvilli)
Staining:High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/118/2084_A_9_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/118/2084_A_4_6.jpg
Normal Tissue
Figure 9 Epididymis
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/118/2084_A_3_6.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000101439-CST3
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Specifications
- Immunogen
- Purified recombinant protein expressed in E. coli.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1
- Specificity
- Cystatin C
- Species Reactivity
- Human
- Clone
- 4A9-8D8-1D5
- Applications
- WB, IHC, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1:1000-1:2000; IHC: 1:100-1:200; ELISA: 1:10000-1:20000
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CST3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1014 | Mouse Anti-CST3 Recombinant Antibody (clone YC029); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-048 | Recombinant Anti-Human CST3 VHH Single Domain Antibody | IHC, FC, CA, FuncS | Llama VHH |
| HPAB-0350-YC-VHH | Recombinant Camel Anti-CST3 Single Domain Antibody (HPAB-0350-YC-VHH) | ELISA | Camel VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0648MZ | Chicken Anti-Human Cystatin C Polyclonal IgY | N/A | Chicken antibody |
| BRD-0649MZ | Chicken Anti-Human Cystatin C complete IgY egg yolk Polyclonal IgY | N/A | Chicken antibody |
| BRD-1127MZ | Chicken Anti-Human Cystatin C Polyclonal IgY | Indirect ELISA | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0844 | Hi-Affi™ Rabbit Anti-CST3 Recombinant Antibody (clone DS844AB) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0425-CN | Rabbit Anti-CST3 Recombinant Antibody (clone CBACN-170) | WB, IHC | Rabbit IgG |
| ZG-0170U | Mouse Anti-CST3 Recombinant Antibody (clone 3A1B7) | ELISA, IHC | Mouse IgG2b |
| VS3-QX289 | Mouse Anti-CST3 Recombinant Antibody (clone 6A4-8C9-4B1) | WB, IHC, ELISA | Mouse IgG1 |
| VS3-QX290 | Mouse Anti-CST3 Recombinant Antibody (clone 7E3-4G2-9C8) | WB, IHC, ELISA | Mouse IgG1 |
| VS3-QX291 | Mouse Anti-CST3 Recombinant Antibody (clone 1H5-4B5-4E4) | WB, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC204 | AbPlus™ Anti-Cst3 Magnetic Beads (VS-0724-YC204) | IP, Protein Purification | |
| VS-0724-YC261 | AbPlus™ Anti-CST3 Magnetic Beads (VS-0724-YC261) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1734 | Anti-CST3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1735 | Anti-Rat CST3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1736 | Anti-Mouse CST3 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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