Anti-CTNNB1 Antibody Prodrug, Protease Activated (13C6)

Name: Anti-CTNNB1 Antibody Prodrug, Protease Activated (13C6)
SKU: VS-1025-YC17
Rating: 5 (1 reviews)

CAT#: VS-1025-YC17

The Anti-CTNNB1 Antibody Prodrug, Protease Activated (13C6) is an antibody prodrug comprised of a recombinant mouse antibody targeting human CTNNB1, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to CTNNB1 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to CTNNB1 effectively. CTNNB1 encodes the protein beta-catenin, which is central to both the structural integrity of cell-to-cell adhesions and the Wnt signaling pathway. Oncogenic mutations in CTNNB1 typically prevent its degradation, leading to its abnormal accumulation in the cytoplasm and nucleus, where it acts as a transcription factor. This nuclear translocation drives the expression of target genes that promote proliferation, resulting in the initiation and progression of cancers like hepatocellular carcinoma and certain types of colorectal tumors.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29159/if_selected.jpg

Normal Tissue Figure 2 IHC staining of human stomach

Immunohistochemical staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29159/ihc_selected.jpg

Normal Tissue Figure 3 IHC staining of human colon

Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/108/ihc_selected.jpg

Normal Tissue Figure 4 IHC staining of human liver

Immunohistochemical staining of human liver shows strong positivity of cytoplasm and nuclei in hepatocytes.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1950/ihc_selected.jpg

Normal Tissue Figure 5 Cerebral cortex

Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_B_8_5.jpg

Normal Tissue Figure 6 Colon

Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_9_3.jpg

Normal Tissue Figure 7 Liver

Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_9_4.jpg

Normal Tissue Figure 8 Pancreas

Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Pancreatic endocrine cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_1_3.jpg

Normal Tissue Figure 9 Kidney

Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_9_5.jpg

Normal Tissue Figure 10 Testis

Sertoli cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_4_6.jpg

RNA Expression Figure 11 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168036-CTNNB1

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Specifications

Host Animal
Mouse

Applications
In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay

Specificity
Human CTNNB1

Target
CTNNB1

Species Reactivity
Human

Isotype
IgG1

Clone
13C6

Masking Approach
Affinity mask

Mask
A synthetic peptide

Protease Cleavable Linker
MMP-cleavable linker

Protease Substrate
MMPs

Purification
Protein G purified

Buffer
PBS, pH 7.4

Preservative
No preservatives

Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Target

Introduction
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.

Alternative Names
Catenin (Cadherin-Associated Protein), Beta 1, 88kDa; CTNNB; Catenin (Cadherin-Associated Protein), Beta 1 (88kD); Beta-Catenin; Armadillo; MRD19

Gene ID
1499

UniProt ID
P35222

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Datasheet

Datasheet - VS-1025-YC17.pdf

MSDS

MSDS - VS-1025-YC17.pdf

COA

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