Rabbit Anti-CTNNB1 Recombinant Antibody (VS13-YC266)
CAT#: VS13-YC266
The Rabbit Anti-CTNNB1 Recombinant Antibody is specific for human, mouse and rat CTNNB1. CTNNB1, β-Catenin, coordinates cellular adhesion and gene transcription, associated with cancers by affecting signaling pathways like Wnt. The anti-CTNNB1 antibody can be used for Western blot (1/500), Immunohistochemistry-paraffin (1/20-1/50), Immunofluorescence (1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29159/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29159/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human colon
Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/108/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human liver
Immunohistochemical staining of human liver shows strong positivity of cytoplasm and nuclei in hepatocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1950/ihc_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_9_4.jpg
Normal Tissue
Figure 8 Pancreas
Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Pancreatic endocrine cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_1_3.jpg
Normal Tissue
Figure 9 Kidney
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_9_5.jpg
Normal Tissue
Figure 10 Testis
Sertoli cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_4_6.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168036-CTNNB1
❮
❯
Specifications
- Immunogen
- Recombinant human Beta-Catenin, aa 30-70.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CTNNB1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, ICC, IF, IP
- Conjugate
- NONE
- Related Disease
- Hepatocellular carcinoma, Colorectal carcinoma, Lung cancer, Malignant breast tumors, Ovarian and Endometrial cancer
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500
Immunohistochemistry-paraffin (IHC-P): 1/20-1/50
Immunofluorescence (IF): 1/100
Target
- Alternative Names
- Catenin Beta 1; Catenin (Cadherin-Associated Protein), Beta 1, 88kDa; CTNNB; Catenin (Cadherin-Associated Protein), Beta 1 (88kD); Catenin (Cadherin-Associated Protein), Beta 1; Catenin Beta-1;
- Gene ID
- 1499
- UniProt ID
- P35222
- Long Name
- Catenin Beta 1
- Sequence Similarities
- Belongs to the beta-catenin family.
- Cellular Localization
- Cell junction; Cell membrane; Cytoplasm; Cytoskeleton; Nucleus; Synapse
- Protein Refseq
- NP_001091679.1; NP_001091680.1; NP_001895.1
- Function
- Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22647378, PubMed:22699938, PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity).
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS13-YC266. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Diseases
Cushing Syndrome
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CTNNB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-247-F(E) | Recombinant Anti-human CTNNB1 Antibody Fab Fragment | ELISA, Nert, FuncS | Fab |
| MHH-247-F(E) | Recombinant Human Anti-human CTNNB1 Antibody Fab Fragment | ELISA, WB, Neut, FuncS | Fab |
| PSBZ-140 | Mouse Anti-CTNNB1 Recombinant Antibody (clone BC-22); scFv Fragment | ELISA, Block | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-247-S(P) | Recombinant Anti-human CTNNB1 Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| MHH-247-S(P) | Recombinant Human Anti-human CTNNB1 Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
| PFBZ-140 | Mouse Anti-CTNNB1 Recombinant Antibody (clone BC-22); Fab Fragment | ELISA, Block | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-049 | Recombinant Anti-Mouse Ctnnb1 VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0859 | Hi-Affi™ Rabbit Anti-CTNNB1 Recombinant Antibody (clone DS859AB) | WB | Rabbit IgG |
| MOR-0039-FY | Rabbit Anti-CTNNB1 Recombinant Antibody (clone AFY0010) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-008CN | Mouse Anti-CTNNB1 Recombinant Antibody (clone 13C6) | ELISA, IHC | Mouse IgG1 |
| NS-008CN-F(E) | Mouse Anti-CTNNB1 Recombinant Antibody (clone 13C6); Fab Fragment | ELISA, IHC | Mouse Fab |
| NS-008CN-S(P) | Mouse Anti-CTNNB1 Recombinant Antibody (clone 13C6); scFv Fragment | ELISA, IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0625J | Rabbit Anti-CTNNB1 Recombinant Antibody (clone 4C11) | ELISA, IF | Rabbit IgG |
| VS3-FY423 | Recombinant Mouse Anti-CTNNB1 Antibody (clone 5B6E9) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS3-FY424 | Recombinant Mouse Anti-CTNNB1 Antibody (clone 9A4-10E6-7G5) | IHC-P | Mouse IgG2b |
| VS3-FY425 | Recombinant Rabbit Anti-CTNNB1 Antibody (clone R07-9D5) | WB, ICC, IF | Rabbit IgG |
| VS3-FY2679 | Rabbit Anti-CTNNB1 Recombinant Antibody (VS3-FY2679) | WB, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX299 | Mouse Anti-CTNNB1 Recombinant Antibody (clone 516CT4.3.3) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY50 | Mouse Anti-NHP CTNNB1 Recombinant Antibody (clone 15B8) | WB, IHC, IF | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY577 | Anti-CTNNB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1770 | Anti-Mouse CTNNB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1769 | Anti-Human CTNNB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1771 | Anti-Sheep CTNNB1 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, WB
Application: FC, FRET, Internalization
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
-3.jpg)
Application: Neut
Application: FC, IHC, Cyt, FuncS
-2.png)
Application: ELISA
Application: ELISA, Cyt, PP, Inhib
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
