Rabbit Anti-CUX1 Recombinant Antibody (clone R03-5F8) (CAT#: VS3-FY2070)
This clone R03-5F8 is a rabbit recombinant monoclonal antibody that is involved in the recognition of human CUX1. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, ICC/IF, IP. The R03-5F8 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20).
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ICC
Figure 1 Anti-CUX1 Antibody (VS3-FY2070) in ICC.
Immunocytochemistry staining of Protein CASP (green) in A549 using the anti-Protein CASP antibody. Nuclear DNA was labeled with DAPI (shown in blue).
WB
Figure 2 Anti-CUX1 Antibody (VS3-FY2070) in WB.
The Protein CASP expression in 3T3, Raw264.7 lysates were detected using the anti-Protein CASP by western blot assay.
Specifications
- Immunogen
- Recombinant human CASP protein protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CUX1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R03-5F8
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 77 kDa, while the observed molecular weight stands at 77 kDa.
- Related Disease
- Fiedler's Myocarditis and Dirofilariasis
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Cut Like Homeobox 1; Golgi Integral Membrane Protein 6; CCAAT Displacement Protein; Homeobox Protein Cux-1; CUTL1; CDP; Cut (Drosophila)-Like 1 (CCAAT Displacement Protein); Cut-Like 1, CCAAT Displacement Protein (Drosophila); Putative Protein Product Of Nbla10317; Cut-Like Homeobox 1; Protein CASP; Cut Homolog; Nbla10317
- Gene ID
- 1523
- UniProt ID
- Q13948
- Sequence Similarities
- Belongs to the CASP family.
- Cellular Localization
- Golgi apparatus, Membrane
- Post Translation Modifications
- Phosphorylated by PKA. (P39880-CUX1_HUMAN)
As cells progress into S phase, a fraction of CUX1 molecules is proteolytically processed into N-terminally truncated proteins of 110 kDa by CTSL.
Cell cycle-dependent processing of CUX1 serves to generate a CDP/Cux p110 with distinct DNA binding and transcriptional properties.
(P39880-CUX1_HUMAN)
Ubiquitination at Lys611 (NX_P39880 [NX_P39880-1])
Modification sites at PhosphoSitePlus (P39880)
Glycosylation from GlyGen (P39880) 5 sites, 1 O-linked glycan (5 sites)
- Protein Refseq
- XP_005250207.2; XP_005250211.2; XP_006715917.2; XP_011514127.1; XP_016867249.1
- Function
- May be involved in intra-Golgi retrograde transport.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3264z | Mouse Anti-CUX1 Recombinant Antibody (clone 7B2) | ELISA, IF, IHC, WB | Mouse IgG1, κ |
| MOB-2059MZ | Recombinant Mouse Anti-Human CUX1 Antibody | FC, ICC/IF, IHC-P, WB | Mouse antibody |
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