Rabbit Anti-CUX1 Recombinant Antibody (clone R03-5F8) (CAT#: VS3-FY2070)
This clone R03-5F8 is a rabbit recombinant monoclonal antibody that is involved in the recognition of human CUX1. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, ICC/IF, IP. The R03-5F8 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20).
Specific Inquiry
ICC
Figure 1 Anti-CUX1 Antibody (VS3-FY2070) in ICC.
Immunocytochemistry staining of Protein CASP (green) in A549 using the anti-Protein CASP antibody. Nuclear DNA was labeled with DAPI (shown in blue).
WB
Figure 2 Anti-CUX1 Antibody (VS3-FY2070) in WB.
The Protein CASP expression in 3T3, Raw264.7 lysates were detected using the anti-Protein CASP by western blot assay.
Specifications
- Immunogen
- Recombinant human CASP protein protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat CUX1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R03-5F8
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 77 kDa, while the observed molecular weight stands at 77 kDa.
- Related Disease
- Fiedler's Myocarditis and Dirofilariasis
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Cut Like Homeobox 1; Golgi Integral Membrane Protein 6; CCAAT Displacement Protein; Homeobox Protein Cux-1; CUTL1; CDP; Cut (Drosophila)-Like 1 (CCAAT Displacement Protein); Cut-Like 1, CCAAT Displacement Protein (Drosophila); Putative Protein Product Of Nbla10317; Cut-Like Homeobox 1; Protein CASP; Cut Homolog; Nbla10317
- Gene ID
- 1523
- UniProt ID
- Q13948
- Sequence Similarities
- Belongs to the CASP family.
- Cellular Localization
- Golgi apparatus, Membrane
- Post Translation Modifications
- Phosphorylated by PKA. (P39880-CUX1_HUMAN)
As cells progress into S phase, a fraction of CUX1 molecules is proteolytically processed into N-terminally truncated proteins of 110 kDa by CTSL.
Cell cycle-dependent processing of CUX1 serves to generate a CDP/Cux p110 with distinct DNA binding and transcriptional properties.
(P39880-CUX1_HUMAN)
Ubiquitination at Lys611 (NX_P39880 [NX_P39880-1])
Modification sites at PhosphoSitePlus (P39880)
Glycosylation from GlyGen (P39880) 5 sites, 1 O-linked glycan (5 sites)
- Protein Refseq
- XP_005250207.2; XP_005250211.2; XP_006715917.2; XP_011514127.1; XP_016867249.1
- Function
- May be involved in intra-Golgi retrograde transport.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3264z | Mouse Anti-CUX1 Recombinant Antibody (clone 7B2) | ELISA, IF, IHC, WB | Mouse IgG1, κ |
| MOB-2059MZ | Recombinant Mouse Anti-Human CUX1 Antibody | FC, ICC/IF, IHC-P, WB | Mouse antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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