Human Anti-CXCR4 Recombinant Antibody (clone Hz515H7-VH1-D76N-VL2) (CAT#: HPAB-1685-FY)

Figure 1 Show the competition of specific [125I]SDF1 binding by 515H7 Mab on cellular membranes of CHO-K1 cells stably expressing wild-type human CXCR4 (T: total binding; NS: non-specific binding).

Figure 2 Shows the modulation of G protein activation by 515H7 Mab by monitoring [35S]GTPγS binding responses at wild-type CXCR4 receptor stably expressed in NIH-3T3 cells.

Figure 3 Show the modulation of CXCR4 receptor association with different interaction partners by SDF-1 and by 515H7 Mab via a bioluminescence resonance energy transfer (BRET) approach in HEK293 cells.

Figure 4 Shows inhibition of MDA-MB-231 xenograft tumor growth by anti-CXCR4 Mab 515H7 in Nod/Scid mice.

Figure 5 Shows the inhibition of SDF-1-induced U937 cells migration by CXCR4 Mab 515H7 in vitro.

Figure 6 Shows the modulation of G protein activation by murine m515H7 Mab and by chimeric c515H7 Mab by monitoring [35S]GTPγS binding responses at wild-type CXCR4 receptor stably expressed in NIH-3T3 cells stimulated with SDF-1 (10 nM).

Figure 7 Shows the modulation of G protein activation by anti-CXCR4 murine m515H7 Mab and chimeric c515H7 Mab by monitoring [35S]GTPγS binding responses at HeLa human tumor cells stimulated with SDF-1 (10 nM).

Figure 8 Show cross blocking of the biotinylated murine antibody 515H7 by the chimeric 515H7 and different variants of the humanized 515H7.

The activity of the humanized variants of 515H7 (hz515H7) to cross block the parental murine antibody 515H7 was evaluated by flow cytometry using CXCR4 transfected NIH3T3 cells.

Figure 9 Show the 515H7 humanized Mabs (hz515H7 VH1 D76N VL2, hz515H7 VH1 D76N VL2.1, hz515H7 VH1 D76N VL2.2 and hz515H7 VH1 D76N VL2.3) specific binding to CXCR4 on NIH3T3-CXCR4 cells.

Figure 10 Show the 515H7 humanized Mabs (hz515H7 VH1 D76N VL2, hz515H7 VH1 D76N VL2.1, hz515H7 VH1 D76N VL2.2 and hz515H7 VH1 D76N VL2.3) specific binding to CXCR4 on Ramos cells.