Human Anti-CXCR4 Recombinant Antibody (clone Hz515H7-VH1-D76N-VL2)

Name: Human Anti-CXCR4 Recombinant Antibody (clone Hz515H7-VH1-D76N-VL2)
SKU: HPAB-1685-FY
Rating: 5 (1 reviews)

CAT#: HPAB-1685-FY

This product is a recombinant antibody that recognizes CXCR4. The monoclonal antibody Hz515H7-VH1-D76N-VL2 reacts with the epitope of CXCR4.

Published Data Gene Expression

RIA

Figure 1 Show the competition of specific [125I]SDF1 binding by 515H7 Mab on cellular membranes of CHO-K1 cells stably expressing wild-type human CXCR4 (T: total binding; NS: non-specific binding).

RIA

Figure 2 Shows the modulation of G protein activation by 515H7 Mab by monitoring [35S]GTPγS binding responses at wild-type CXCR4 receptor stably expressed in NIH-3T3 cells.

Figure 3 Show the modulation of CXCR4 receptor association with different interaction partners by SDF-1 and by 515H7 Mab via a bioluminescence resonance energy transfer (BRET) approach in HEK293 cells.

Inhib

Figure 4 Shows inhibition of MDA-MB-231 xenograft tumor growth by anti-CXCR4 Mab 515H7 in Nod/Scid mice.

Inhib

Figure 5 Shows the inhibition of SDF-1-induced U937 cells migration by CXCR4 Mab 515H7 in vitro.

FuncS

Figure 6 Shows the modulation of G protein activation by murine m515H7 Mab and by chimeric c515H7 Mab by monitoring [35S]GTPγS binding responses at wild-type CXCR4 receptor stably expressed in NIH-3T3 cells stimulated with SDF-1 (10 nM).

FuncS

Figure 7 Shows the modulation of G protein activation by anti-CXCR4 murine m515H7 Mab and chimeric c515H7 Mab by monitoring [35S]GTPγS binding responses at HeLa human tumor cells stimulated with SDF-1 (10 nM).

Block

Figure 8 Show cross blocking of the biotinylated murine antibody 515H7 by the chimeric 515H7 and different variants of the humanized 515H7.

The activity of the humanized variants of 515H7 (hz515H7) to cross block the parental murine antibody 515H7 was evaluated by flow cytometry using CXCR4 transfected NIH3T3 cells.

Figure 9 Show the 515H7 humanized Mabs (hz515H7 VH1 D76N VL2, hz515H7 VH1 D76N VL2.1, hz515H7 VH1 D76N VL2.2 and hz515H7 VH1 D76N VL2.3) specific binding to CXCR4 on NIH3T3-CXCR4 cells.

Figure 10 Show the 515H7 humanized Mabs (hz515H7 VH1 D76N VL2, hz515H7 VH1 D76N VL2.1, hz515H7 VH1 D76N VL2.2 and hz515H7 VH1 D76N VL2.3) specific binding to CXCR4 on Ramos cells.

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RNA Expression Figure 1 RNA cell line category: Cell line enhanced (Daudi, JURKAT, Karpas-707, MOLT-4, REH, U-266/70, U-698)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000121966-CXCR4

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Specifications

Host Species
Human

Derivation
Humanized

Type
Humanized IgG1

Specificity
Human CXCR4

Species Reactivity
Human

Clone
Hz515H7-VH1-D76N-VL2

Applications
ELISA, FC

Related Disease
Cancer

Product Property

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
The CXCR4 antibody has been reported in applications of Radioimmunoassay, Immunoassay, Inhibition, Functional Assay, Blocking, Flow Cytometry.

Target

Alternative Names
C-X-C Motif Chemokine Receptor 4; Leukocyte-Derived Seven Transmembrane Domain Receptor; Lipopolysaccharide-Associated Protein 3; Stromal Cell-Derived Factor 1 Receptor; Chemokine (C-X-C Motif) Receptor 4; LPS-Associated Protein 3; SDF-1 Receptor; CD184 Antigen; Fusin; LAP-3; LESTR; NPYRL; FB22; HM89; LCR1; Seven-Transmembrane-Segment Receptor, Spleen; Chemokine (C-X-C Motif), Receptor 4 (Fusin)

Gene ID
7852

UniProt ID
P61073

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-1685-FY.pdf

MSDS

MSDS - HPAB-1685-FY.pdf

COA

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