Rabbit Anti-CYB5R3 Recombinant Antibody (clone R01-9F3)
CAT#: VS3-FY1690
Clone R01-9F3 is a rabbit recombinant monoclonal antibody that is involved in the recognition of human CYB5R3. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-P. This CYB5R3 antibody has been teted positive by western blot analysis of CYB5R3 in C6, Hela lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1566/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1566/5328_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1566/5328_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1566/5328_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1566/5328_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1566/5328_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1566/5328_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (ASC diff)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100243-CYB5R3
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WB
Figure 1 Anti-CYB5R3 Antibody (VS3-FY1690) in WB.
The CYB5R3 expression in C6, Hela lysates were detected using the anti-CYB5R3 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human CYB5R3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat CYB5R3
- Species Reactivity
- Human, Rat
- Clone
- R01-9F3
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 34 kDa, while the observed molecular weight stands at 34 kDa.
- Related Disease
- Methemoglobinemia Due To Deficiency Of Methemoglobin Reductase and Hereditary Methemoglobinemia
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Cytochrome B5 Reductase 3; NADH-Cytochrome B5 Reductase 3; Diaphorase-1; EC 1.6.2.2; DIA1; B5R
- Gene ID
- 1727
- UniProt ID
- P00387
- Sequence Similarities
- Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
- Cellular Localization
- Cytoplasm, Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane
- Post Translation Modifications
- Acetylation
Lipoprotein
Myristate
Phosphoprotein
- Protein Refseq
- XP_047297139.1; XP_054181199.1; NP_000389.1; NP_001123291.1; NP_001165131.1
- Function
- Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CYB5R3"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1807z | Mouse Anti-CYB5R3 Recombinant Antibody (clone 7B12) | WB, FC, IHC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0893 | Hi-Affi™ Rabbit Anti-CYB5R3 Recombinant Antibody (clone DS893AB) | IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1113 | AbPlus™ Anti-CYB5R3 Magnetic Beads (VS-0724-YC1113) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY597 | Anti-CYB5R3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1834 | Anti-Human CYB5R3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1835 | Anti-Rat CYB5R3 Immunohistochemistry Kit | IHC |
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