Mouse Anti-CYP1A1 Recombinant Antibody (VS3-CJ382) (CAT#: VS3-CJ382)

• Immunogen
• KLH-conjugated synthetic peptide between 377-405 amino acids from the C-terminal region of human CYP1A1

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human CYP1A1

• Species Reactivity
• Human

• Applications
• WB

• Conjugate
• Unconjugated

• Purification
• Protein G affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• PBS

• Preservative
• 0.09% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Western Blot (1:1000).

• Alternative Names
• AHH; AHRR; CP11; CYP1; CYPIA1; P1-450; P450-C; P450DX

• Gene ID
• 1543

• UniProt ID
• P04798

• Sequence Similarities
• Belongs to the cytochrome P450 family.

• Cellular Localization
• Cytoplasm, Endoplasmic reticulum, Membrane, Microsome

• Protein Refseq
• NP_000490.1; NP_001306145.1; NP_001306146.1

• Function
• A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed:11555828, PubMed:14559847, PubMed:12865317, PubMed:15805301, PubMed:15041462, PubMed:18577768, PubMed:19965576, PubMed:20972997, PubMed:10681376).
  Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:11555828, PubMed:14559847, PubMed:12865317, PubMed:15805301, PubMed:15041462, PubMed:18577768, PubMed:19965576, PubMed:20972997, PubMed:10681376).
  Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C15-alpha and C16-alpha positions (PubMed:11555828, PubMed:14559847, PubMed:12865317, PubMed:15805301).
  Displays different regioselectivities for polyunsaturated fatty acids (PUFA) hydroxylation (PubMed:15041462, PubMed:18577768).
  Catalyzes the epoxidation of double bonds of certain PUFA (PubMed:15041462, PubMed:19965576, PubMed:20972997).
  Converts arachidonic acid toward epoxyeicosatrienoic acid (EET) regioisomers, 8,9-, 11,12-, and 14,15-EET, that function as lipid mediators in the vascular system (PubMed:20972997).
  Displays an absolute stereoselectivity in the epoxidation of eicosapentaenoic acid (EPA) producing the 17(R),18(S) enantiomer (PubMed:15041462).
  May play an important role in all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed:10681376).
  May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent) (PubMed:21068195).

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Secondary Antibody

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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