Mouse Anti-CYP27B1 Recombinant Antibody (VS3-CJ141) (CAT#: VS3-CJ141)

• Immunogen
• KLH-conjugated synthetic peptide between 477-508 amino acids from the C-terminal region of human CYP27B1

• Host Species
• Mouse

• Type
• Mouse IgG1, κ

• Specificity
• Human CYP27B1

• Species Reactivity
• Human

• Applications
• WB

• Conjugate
• Unconjugated

• Purification
• Protein A/G affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• PBS

• Preservative
• 0.09% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Western Blot (1:1000).

• Alternative Names
• VDR; CP2B; CYP1; PDDR; VDD1; VDDR; VDDRI; CYP27B; P450c1; CYP1alpha

• Gene ID
• 1594

• UniProt ID
• O15528

• Sequence Similarities
• Belongs to the cytochrome P450 family.

• Cellular Localization
• Membrane, Mitochondrion

• Protein Refseq
• NP_000776.1

• Function
• A cytochrome P450 monooxygenase involved in vitamin D metabolism and in calcium and phosphorus homeostasis. Catalyzes the rate-limiting step in the activation of vitamin D in the kidney, namely the hydroxylation of 25-hydroxyvitamin D3/calcidiol at the C1alpha-position to form the hormonally active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3/calcitriol that acts via the vitamin D receptor (VDR) (PubMed:10518789, PubMed:9486994, PubMed:22862690, PubMed:10566658, PubMed:12050193).
  Has 1alpha-hydroxylase activity on vitamin D intermediates of the CYP24A1-mediated inactivation pathway (PubMed:10518789, PubMed:22862690).
  Converts 24R,25-dihydroxyvitamin D3/secalciferol to 1-alpha,24,25-trihydroxyvitamin D3, an active ligand of VDR. Also active on 25-hydroxyvitamin D2 (PubMed:10518789).
  Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin (PubMed:22862690).

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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