Human Anti-Cytokeratin K8/K18 Recombinant Antibody (clone COU-1) (CAT#: HPAB-0047LY)
This product is antibody recognizes Cytokeratin K8/K18. The antibody COU-1 can be used in some immunoassay techniques such as: WB.
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ELISA
Figure 1 Binding of COU-1 to recombinant K8/K18 complexes evaluated by ELISA.
Heterotypic complexes, generated by combining purified recombinant K8-(1–129) or intact K8 with purified recombinant K18-(1–124) or intact K18 in equimolar ratios, were coated at 5 g/ml on ELISA plates and incubated with serial dilutions of COU-1.
Ditzel, H. J., Strik, M. C., Larsen, M. K., Willis, A. C., Waseem, A., Kejling, K., & Jensenius, J. C. (2002). Cancer-associated cleavage of cytokeratin 8/18 heterotypic complexes exposes a neoepitope in human adenocarcinomas. Journal of Biological Chemistry, 277(24), 21712-21722.
WB
Figure 2 Purification of cytokeratin from colon cancer tissue.
Western blot-stained with COU-1.
Ditzel, H. J., Strik, M. C., Larsen, M. K., Willis, A. C., Waseem, A., Kejling, K., & Jensenius, J. C. (2002). Cancer-associated cleavage of cytokeratin 8/18 heterotypic complexes exposes a neoepitope in human adenocarcinomas. Journal of Biological Chemistry, 277(24), 21712-21722.
IF
Figure 3 Subcellular distribution of N-terminally truncated K8/K18 recognized by COU-1 and intact K18 recognized by a murineanti-K18 antibody.
Bound COU-1 wasdetected with FITC-goat anti-human-chain antibody (green) and bound anti-K18 with Texas Red goat anti-mouse IgG antibody (red).
Ditzel, H. J., Strik, M. C., Larsen, M. K., Willis, A. C., Waseem, A., Kejling, K., & Jensenius, J. C. (2002). Cancer-associated cleavage of cytokeratin 8/18 heterotypic complexes exposes a neoepitope in human adenocarcinomas. Journal of Biological Chemistry, 277(24), 21712-21722.
IF
Figure 4 Subcellular distribution of N-terminal-truncated K8/K18 recognized by COU-1 and K18 recognized by a murine anti-K18antibody in MCF7 breast cancer cells.
Differential interference contrast images were included to visualize the composition of the cells. Whereas whole intermediate filamentswere stained with anti-K18 (arrowheads), COU-1 (arrows) only stained short fibrils and globular structures.
Ditzel, H. J., Strik, M. C., Larsen, M. K., Willis, A. C., Waseem, A., Kejling, K., & Jensenius, J. C. (2002). Cancer-associated cleavage of cytokeratin 8/18 heterotypic complexes exposes a neoepitope in human adenocarcinomas. Journal of Biological Chemistry, 277(24), 21712-21722.
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human Cytokeratin K8/K18
- Species Reactivity
- Human
- Clone
- COU-1
- Applications
- WB
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The Cytokeratin K8/K18 antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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ScFv Antibody Products
Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0047LY-S(P) | Human Anti-Cytokeratin K8/K18 Recombinant Antibody (clone COU-1); scFv Fragment | WB | Human scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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