Rabbit Anti-DBH Recombinant Antibody (clone R48-3P-5) (CAT#: VS3-FY2194)
This dopamine beta hydroxylase antibody is a rabbit recombinant monoclonal antibody that specifically created to bind human DBH. It has been tested in WB and IHC assay. The R48-3P-5 is recommended for dilution of target protein by western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/100-1/200).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SH-SY5Y
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5960/1669_D6_1_selected.jpg
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5960/1669_D6_1_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70789/1378_H2_4_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70789/1378_H2_4_selected.jpg
Figure 3 Adrenal gland
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2130/160606_B_4_5_val_selected.jpg
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2130/160606_B_4_5_val_selected.jpg
Figure 4 Colon
(Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70789/160599_A_7_3.jpg
(Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70789/160599_A_7_3.jpg
Figure 5 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70789/160599_A_9_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70789/160599_A_9_4.jpg
Figure 6 RNA cell line category: Cell line enriched (SH-SY5Y)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000123454-DBH
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000123454-DBH
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human DBH
- Species Reactivity
- Human
- Clone
- R48-3P-5
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 69 kDa, while the observed molecular weight stands at 70-75kDa.
- Related Disease
- Orthostatic Hypotension 1 and Dopamine Beta-Hydroxylase Deficiency
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Target
- Alternative Names
- Dopamine Beta-Hydroxylase; Dopamine Beta-Hydroxylase (Dopamine Beta-Monooxygenase); Dopamine Beta-Monooxygenase; EC 1.14.17.1; DBM
- Gene ID
- 1621
- UniProt ID
- P09172
- Sequence Similarities
- Belongs to the copper type II ascorbate-dependent monooxygenase family.
- Cellular Localization
- Cytoplasmic vesicle, Membrane, Secreted
- Post Translation Modifications
- N-glycosylated. (P09172-DOPO_HUMAN)
Proteolytic cleavage after the membrane-anchor leads to the release of the soluble form. (P09172-DOPO_HUMAN)
Glycosylation at Asn64, Asn184, and Asn566 (NX_P09172 [NX_P09172-1])
Modification sites at PhosphoSitePlus (P09172)
Glycosylation from GlyConnect DOPO_HUMAN (1933)
Glycosylation from GlyGen (P09172) 4 sites, 9 N-linked glycans (1 site)
- Protein Refseq
- NP_000778.3
- Function
- Catalyzes the hydroxylation of dopamine to noradrenaline (also known as norepinephrine), and is thus vital for regulation of these neurotransmitters.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "DBH"
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IgG Antibody Products
Immunotoxin Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2334z | Mouse Anti-DBH Recombinant Antibody (clone 25D3) | WB, ELISA, IF, IP | Mouse IgG1, κ |
| MOB-0592CT | Recombinant Mouse anti-Rat DBH Monoclonal antibody (9.G.405) | ELISA, ICC, RID, WB |
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