Mouse Anti-DDIT3 Recombinant Antibody (clone 2B1) (CAT#: ZG-0146J)

This product is a mouse antibody that recognizes DDIT3.


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IF

Figure 1 Mouse Anti-DDIT3 Recombinant Antibody (clone 2B1) in IF

Figure 1 Mouse Anti-DDIT3 Recombinant Antibody (clone 2B1) in IF

Immunofluorescence analysis of Hela cell. 1.Calnexin Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min).

IF

Figure 2 Mouse Anti-DDIT3 Recombinant Antibody (clone 2B1) in IF

Figure 2 Mouse Anti-DDIT3 Recombinant Antibody (clone 2B1) in IF

Immunofluorescence analysis of Mouse-brain tissue. 1.CHOP Mouse Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary Antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI.Picture C: merge of A+B

IHC

Figure 3 Mouse Anti-DDIT3 Recombinant Antibody (clone 2B1) in IHC

Figure 3 Mouse Anti-DDIT3 Recombinant Antibody (clone 2B1) in IHC

Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1.CHOP Mouse Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.

IHC

Figure 4 Mouse Anti-DDIT3 Recombinant Antibody (clone 2B1) in IHC

Figure 4 Mouse Anti-DDIT3 Recombinant Antibody (clone 2B1) in IHC

Immunohistochemical analysis of paraffin-embedded Human Stomach Carcinoma Tissue using CHOP Mouse mAb diluted at 1:200.

WB

Figure 5 Mouse Anti-DDIT3 Recombinant Antibody (clone 2B1) in WB

Figure 5 Mouse Anti-DDIT3 Recombinant Antibody (clone 2B1) in WB

Western blot analysis of Mouse Liver Tissue Lysate using CHOP Mouse mAb diluted at 1:2000.


Specifications

  • Immunogen
  • Synthetic Peptide of CHOP at AA range of 10-90
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human DDIT3
  • Species Reactivity
  • Human, Rat, Mouse
  • Clone
  • 2B1
  • Applications
  • WB, IF/ICC, IHC-P

Product Property

  • Storage
  • Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody was validated for Western Blot, Immunofluorescence/Immunocytochemistry, Immunohistochemistry-Paraffin.

Target

  • Alternative Names
  • CHOP; CEBPZ; CHOP10; CHOP-10; GADD153; AltDDIT3; C/EBPzeta; DNA damage-inducible transcript 3 protein; C/EBP zeta; CCAAT/enhancer-binding protein homologous protein; alternative DDIT3 protein; c/EBP-homologous protein 10; growth arrest and DNA damage-inducible protein GADD153

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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