Rabbit Anti-DDX58 Recombinant Antibody (clone 7F6)
CAT#: ZG-0056U
The purified Rabbit Anti-DDX58 Recombinant Antibody (clone 7F6) displayed high specificity against the DDX58 protein of human.
Tested Data
Gene Expression
IHC
Figure 1 Rabbit Anti-DDX58 Antibody (ZG-0056U) in IHC
IHC image of ZG-0056U diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47193/838_D11_7_selected.jpg
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Specifications
- Immunogen
- A synthesized peptide derived from human DDX58
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human DDX58
- Species Reactivity
- Human
- Clone
- 7F6
- Applications
- ELISA, IHC
- Conjugate
- Unconjugated
- Related Disease
- Singleton-Merten Syndrome 2 and Singleton-Merten Syndrome
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunohistochemistry. Specific conditions should be optimized by the end user.
IHC:1:50-1:200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "DDX58"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2347z | Mouse Anti-DDX58 Recombinant Antibody (clone 32G12) | WB, ICC, IF, IHC | Mouse IgG2a |
| VS13-YC296 | Rabbit Anti-DDX58 Recombinant Antibody (VS13-YC296) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4727 | Hi-Affi™ Rabbit Anti-DDX58 Recombinant Antibody (clone TH241DS) | WB | Rabbit IgG |
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