Rabbit Anti-DDX58 Recombinant Antibody (VS13-YC296) (CAT#: VS13-YC296)
The Rabbit Anti-DDX58 Recombinant Antibody is specific for human DDX58. DDX58, a DEAD box helicase, participates in RNA recognition and immune regulation, particularly in recognizing viral RNA and regulating host immune responses. The anti-DDX58 antibody can be used for Western blot (1/500-1/1000), Immunoprecipitation (1/50).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/47193/838_D11_7_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/47193/838_D11_7_selected.jpg
Figure 2 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73217/170537_A_6_8_selected.jpg
(Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73217/170537_A_6_8_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12643/28581_B_8_5.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12643/28581_B_8_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12643/28581_A_9_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12643/28581_A_9_3.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12643/28581_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12643/28581_A_8_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12643/28581_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12643/28581_A_6_6.jpg
Figure 7 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12643/28581_A_9_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/12643/28581_A_9_8.jpg
Figure 8 RNA cell line category: Group enriched (BJ hTERT+ SV40 Large T+, BJ hTERT+ SV40 Large T+ RasG12V, GAMG)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107201-DDX58
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107201-DDX58
Specifications
- Immunogen
- Recombinant human DDX58.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human DDX58
- Species Reactivity
- Human
- Applications
- WB, IP
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4) containing 150 mM NaCl, 40% Glycerol and 0.05% BSA.
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunoprecipitation (IP): 1/50
Target
- Alternative Names
- DExD/H-Box Helicase 58; DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58; Retinoic Acid-Inducible Gene 1 Protein; Retinoic Acid-Inducible Gene I Protein; DEAD Box Protein 58; RNA Helicase RIG-I; RIG-I; RLR-1; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide;
- Gene ID
- 23586
- UniProt ID
- O95786
- Long Name
- DExD/H-Box Helicase 58
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibodies
Rabbit Monoclonal Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2347z | Mouse Anti-DDX58 Recombinant Antibody (clone 32G12) | WB, ICC, IF, IHC | Mouse IgG2a |
| ZG-0056U | Rabbit Anti-DDX58 Recombinant Antibody (clone 7F6) | ELISA, IHC | Rabbit IgG |
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