Mouse Anti-DENV NS1 Recombinant Antibody (clone 8A6F2) (CAT#: FAMAB-0149WJ)
This recombinant monoclonal antibody was derived from a positive hybridoma clone carried out by a CELLine 1000 bioreactor. It is a DENV-4-specific anti-NS1 MAb, characterized using fixed cell ELISA and iELISA. This antibody can be used in applications, such as ELISA, western blot, and Neutralization. A matching pair of unbiotinylated 8A6F2 as a capture antibody and biotinylated 6D4B10 as a detection antibody provided the most robust competition ELISA results.
Specific Inquiry
WB
Figure 1 Anti-NS1 Monoclonal Antibodies (MAbs) reactivity to SUMO*-NS1 fusion protein.
Western blot assay to determine the reactivity of hybridoma supernatants to SUMO*-NS1 fusion protein as well as unfused commercial rNS1 protein expressed in mammalian cell.
Gelanew T, Hunsperger E. Development and characterization of serotype-specific monoclonal antibodies against the dengue virus-4 (DENV-4) non-structural protein (NS1). Virol J. 2018 Feb 6;15(1):30.
ELISA
Figure 2 Serotype-specificity of monoclonal antibodies (MAbs 3H7A9, 8A6F2) against DENV-4 determined by iELISA.
The bars show mean optical density (OD) at 450 nm which measures MAb reactivity to NS1s to all four DENV-4 serotypes, yellow fever virus (YFV), West Nile virus (WNV) and UMO protein.
Gelanew T, Hunsperger E. Development and characterization of serotype-specific monoclonal antibodies against the dengue virus-4 (DENV-4) non-structural protein (NS1). Virol J. 2018 Feb 6;15(1):30.
Specifications
- Immunogen
- Recombinant (r) flavivirus NS1 antigens
- Host Species
- Mouse
- Derivation
- Hybridoma
- Type
- Mouse IgG2b, κ
- Specificity
- NS1 DENV-4
- Species Reactivity
- DENV
- Clone
- 8A6F2
- Applications
- ELISA, WB, Neut
- Related Disease
- DENV infection
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Dengue virus; DENV
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0263MC | Rabbit Anti-Dengue virus NS1 Antibody | ICC/IF, WB | |
| MOB-0266MC | Recombinant Anti-Dengue virus NS1 protein Antibody | ICC/IF, WB | |
| VS-0423-CJ5 | Human Anti-DENV NS1 Recombinant Antibody (clone R2.NS1_E8) | WB, ELISA, FC | Human IgG1 |
| VS-0423-CJ6 | Human Anti-DENV NS1 Recombinant Antibody (clone R3.NS1_H6) | WB, ELISA, FC | Human IgG1 |
| VS-0423-CJ7 | Human Anti-DENV NS1 Recombinant Antibody (clone R3.NS1_A8) | WB, ELISA, FC | Human IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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