Mouse Anti-Desipramine Recombinant Antibody (clone B9) (CAT#: FAMAB-0064CQ)

Figure 1 Mouse Anti-Desipramine Antibody (clone B9) in SDS-PAGE.

Nom-educing SDS-PAGE of (A) B9-IgG (150 kDa), (B) B9-Fab (50 kDa), and (C) B9-sFv (27 kDa). It is common for sFv fragments to migrate on SDS-PAGE with an apparent size that is slightly higher than calculated [26]. The 35 kDa band associated with Fab has not been identified.

Shelver, W. L., Keyler, D. E., Lin, G., Murtaugh, M. P., Flickinger, M. C., Ross, C. A., & Pentel, P. R. (1996). Effects of recombinant drug-specific single chain antibody Fv fragment on [3H]-desipramine distribution in rats. Biochemical pharmacology, 51(4), 531-537.

Figure 2 Mouse Anti-Desipramine Antibody (clone B9) in ELISA.

(A) Stability of B9-sFv at 37°C and a concentration of 0.2 mg/mL in various solutions. (B) Stability of BB-sFv at 37°C, at concentrations corresponding to those typically found in each solution in the current study; 2.4 mg/mL in elution buffer, 0.2 mg/mL in serum, and 0.02 mg/mL in medium. N = 6 for both panels A and B. (C) Stability of sFv at lower temperatures (data represent single samples at each time point). Note that the time scale differs from that of A and B, and that SFV activity was measured as the ELISA absorbance ratio rather than as concentrations.

Shelver, W. L., Keyler, D. E., Lin, G., Murtaugh, M. P., Flickinger, M. C., Ross, C. A., & Pentel, P. R. (1996). Effects of recombinant drug-specific single chain antibody Fv fragment on [3H]-desipramine distribution in rats. Biochemical pharmacology, 51(4), 531-537.