Mouse Anti-Desipramine Recombinant Antibody (clone G5) (CAT#: FAMAB-0066CQ)

Figure 1 Stability of G5-sFv.

Affinity purified G5-sFv was diluted to 0.1 mg/ml in elution buffer (5% acetonitrile, 1% propionic acid, pH 4.5) (●), rat serum (○), or rat urine (□), and incubated at 37 centigrade for various lengths of time. 100 μg of desipramine-BSA conjugate was placed into wells of a 96 well plate at 4 centigrade overnight and washed in PBS with 0.2% Tween-20. G5-sFv samples, after each treatment, were incubated in triplicate wells for 2 h. Chicken anti-G5-Fab' polyclonal antibody and rabbit anti-chicken IgG peroxidase conjugate were used to detect and quantify bound G5-sFvs. The absorbance at 492 nm of each sample was determined using a microplate reader.

Lin, G., Pentel, P. R., Shelver, W. L., Keyler, D. E., Ross, C. A., Hieda, Y., ... & Murtaugh, M. P. (1996). Bacterial expression and characterization of an anti-desipramine single-chain antibody fragment. International journal of immunopharmacology, 18(12), 729-738.

Figure 2 In vivo redistribution of desipramine by anti-G5 antibody types.

Redistribution of labeled DMI in plasma was determined as the percentage increase in radioactivity over time. Data represent the mean value from five animals at each time point.

Lin, G., Pentel, P. R., Shelver, W. L., Keyler, D. E., Ross, C. A., Hieda, Y., ... & Murtaugh, M. P. (1996). Bacterial expression and characterization of an anti-desipramine single-chain antibody fragment. International journal of immunopharmacology, 18(12), 729-738.