Rabbit Anti-DHX9 Recombinant Antibody (clone R03-8A8)
CAT#: VS3-FY1871
This rabbit recombinant monoclonal antibody has been developed for the specific purpose of detecting human and rat DHX9. The immunogen is a synthetic peptide of human RNA Helicase A. The calculated molecular weight of the protein is 141 kDa, with an observed molecular weight also at 141 kDa. For western blot applications, the recommended dilution range is 1/500 to 1/1000.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28050/299_H11_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/55684/if_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11819/637_H3_2_selected.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28050/59424_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28050/59424_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28050/59424_A_9_8.jpg
Normal Tissue
Figure 7 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28050/59424_B_7_5.jpg
Normal Tissue
Figure 8 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28050/59424_A_7_3.jpg
Normal Tissue
Figure 9 Pancreas
Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Pancreatic endocrine cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28050/59424_A_3_3.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135829-DHX9
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Specifications
- Immunogen
- Synthetic peptide of human RNA helicase A
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat DHX9
- Species Reactivity
- Human, Rat
- Clone
- R03-8A8
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 141 kDa, while the observed molecular weight stands at 141 kDa.
- Related Disease
- Werner Syndrome and Chikungunya
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- DExH-Box Helicase 9; Nuclear DNA Helicase II; RNA Helicase A; Leukophysin; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 9; DEAH (Asp-Glu-Ala-His) Box Helicase 9; DEAH Box Protein 9; NDH II; DDX9; NDH2
- Gene ID
- 1660
- UniProt ID
- Q08211
- Cellular Localization
- Cytoplasm, Cytoskeleton, Nucleus
- Post Translation Modifications
- Methylated (PubMed:15084609).
PRMT1-mediated methylation of undefined Arg residues in the RGG region is required for nuclear import of DHX9.
- Protein Refseq
- NP_001348.2
- Function
- Plays a role in DNA replication at origins of replication and cell cycle progression.
Plays a role as a transcriptional coactivator acting as a bridging factor between polymerase II holoenzyme and transcription factors or cofactors, such as BRCA1, CREBBP, RELA and SMN1
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "DHX9"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3960z | Mouse Anti-DHX9 Recombinant Antibody (clone 26H2) | ELISA, IF, IHC, WB | Mouse IgG1, κ |
| MOB-1286MZ | Recombinant Mouse Anti-Human DHX9 Antibody (clone 3G7) | ICC, IF, IHC-P, IP, WB | Mouse antibody |
| VS13-YC302 | Rabbit Anti-DHX9 Recombinant Antibody (VS13-YC302) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0985 | Hi-Affi™ Rabbit Anti-DHX9 Recombinant Antibody (clone DS985AB) | WB, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY660 | Anti-DHX9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1990 | Anti-Mouse DHX9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1989 | Anti-Human DHX9 Immunohistochemistry Kit | IHC |
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