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    Rabbit Anti-DHX9 Recombinant Antibody (clone R03-8A8) (CAT#: VS3-FY1871)

    This rabbit recombinant monoclonal antibody has been developed for the specific purpose of detecting human and rat DHX9. The immunogen is a synthetic peptide of human RNA Helicase A. The calculated molecular weight of the protein is 141 kDa, with an observed molecular weight also at 141 kDa. For western blot applications, the recommended dilution range is 1/500 to 1/1000.

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    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line A-431
    Figure 1 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/28050/299_H11_2_selected.jpg
    Figure 2 IF staining of human cell line U-2 OS
    Figure 2 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/55684/if_selected.jpg
    Figure 3 IF staining of human cell line U-2 OS
    Figure 3 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/11819/637_H3_2_selected.jpg
    Normal Tissue
    Figure 4 Kidney
    Figure 4 Kidney
    (Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/28050/59424_A_7_5.jpg
    Figure 5 Testis
    Figure 5 Testis
    (Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/28050/59424_A_4_6.jpg
    Figure 6 Lymph node
    Figure 6 Lymph node
    (Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/28050/59424_A_9_8.jpg
    Figure 7 Cerebral cortex
    Figure 7 Cerebral cortex
    (Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/28050/59424_B_7_5.jpg
    Figure 8 Colon
    Figure 8 Colon
    (Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/28050/59424_A_7_3.jpg
    Figure 9 Pancreas
    Figure 9 Pancreas
    (Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Pancreatic endocrine cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/28050/59424_A_3_3.jpg
    RNA Expression
    Figure 10 RNA cell line category: Low cell line specificity
    Figure 10 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135829-DHX9

    Specifications

    • Immunogen
    • Synthetic peptide of human RNA helicase A
    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human and Rat DHX9
    • Species Reactivity
    • Human, Rat
    • Clone
    • R03-8A8
    • Applications
    • Western Blot
    • MW
    • The calculated weight of the target protein is approximately 141 kDa, while the observed molecular weight stands at 141 kDa.
    • Related Disease
    • Werner Syndrome and Chikungunya

    Product Property

    • Purification
    • Affinity Purified
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • Western Blot: 1/500-1/1000

    Target

    • Alternative Names
    • DExH-Box Helicase 9; Nuclear DNA Helicase II; RNA Helicase A; Leukophysin; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 9; DEAH (Asp-Glu-Ala-His) Box Helicase 9; DEAH Box Protein 9; NDH II; DDX9; NDH2
    • Cellular Localization
    • Cytoplasm, Cytoskeleton, Nucleus
    • Post Translation Modifications
    • Methylated (PubMed:15084609).
      PRMT1-mediated methylation of undefined Arg residues in the RGG region is required for nuclear import of DHX9.
    • Function
    • Plays a role in DNA replication at origins of replication and cell cycle progression.
      Plays a role as a transcriptional coactivator acting as a bridging factor between polymerase II holoenzyme and transcription factors or cofactors, such as BRCA1, CREBBP, RELA and SMN1

    Recommended Products

    Isotype Control

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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    MOB-3960z Mouse Anti-DHX9 Recombinant Antibody (clone 26H2) ELISA, IF, IHC, WB Mouse IgG1, κ
    MOB-1286MZ Recombinant Mouse Anti-Human DHX9 Antibody (clone 3G7) ICC/IF, IHC-P, IP, WB Mouse antibody
    VS13-YC302 Rabbit Anti-DHX9 Recombinant Antibody (VS13-YC302) WB Rabbit IgG

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