Rabbit Anti-DKK3 Recombinant Antibody (clone R03-8G7)
CAT#: VS3-FY1698
This anti-DKK3 antibody detected in western blot analysis of Dkk3 in rat brain lysates. This clone R03-8G7 is a rabbit recombinant monoclonal antibody that is created to detect human and rat DKK3. It is suitable for use in a variety of assays, including WB, IP.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11868/89_C8_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11868/28075_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11868/28075_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11868/28075_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11868/28075_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11868/28075_A_5_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, fHDF/TERT166, hTERT-RPE1, TIME)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000050165-DKK3
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WB
Figure 1 Anti-DKK3 Antibody (VS3-FY1698) in WB.
The Dkk3 expression in rat Brain lysates were detected using the anti-DKK3 by western blot assay.
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Specifications
- Immunogen
- Recombinant human Dkk3 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat DKK3
- Species Reactivity
- Human, Rat
- Clone
- R03-8G7
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 38 kDa, while the observed molecular weight stands at 70 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Dickkopf WNT Signaling Pathway Inhibitor 3; Regulated In Glioma; Dickkopf-3; HDkk-3; Dkk-3; REIC; Dickkopf (Xenopus Laevis) Homolog 3; Dickkopf 3 Homolog (Xenopus Laevis)
- Gene ID
- 27122
- UniProt ID
- Q9UBP4
- Sequence Similarities
- Belongs to the dickkopf family.
- Cellular Localization
- Secreted
- Post Translation Modifications
- N- and O-glycosylated.
- Protein Refseq
- XP_006718241.1; XP_016873044.1; XP_047282730.1; XP_047282731.1; NP_001018067.1
- Function
- Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).
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Related Signaling Pathways
Canonical Wnt Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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