Rabbit Anti-DKK3 Recombinant Antibody (VS13-YC304)
CAT#: VS13-YC304
The Rabbit Anti-DKK3 Recombinant Antibody is specific for human, mouse and rat DKK3. DKK3 is a secreted protein implicated in embryonic development through Wnt pathway interactions, potentially functioning as a tumor suppressor with variant isoforms. The anti-DKK3 antibody can be used for Western blot (1/500-1/1000), Immunofluorescence (1/50-1/200), Immunoprecipitation (1/50).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11868/89_C8_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11868/28075_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11868/28075_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11868/28075_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11868/28075_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11868/28075_A_5_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, fHDF/TERT166, hTERT-RPE1, TIME)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000050165-DKK3
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Specifications
- Immunogen
- Recombinant human Dkk3.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat DKK3
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IP
- Conjugate
- NONE
- Related Disease
- Cancer
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4) containing 150 mM NaCl, 40% Glycerol and 0.05% BSA.
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunofluorescence (IF): 1/50-1/200
Immunoprecipitation (IP): 1/50
Target
- Alternative Names
- Dickkopf WNT Signaling Pathway Inhibitor 3; Regulated In Glioma; Dickkopf-3; HDkk-3; Dkk-3; REIC; Dickkopf (Xenopus Laevis) Homolog 3; Dickkopf 3 Homolog (Xenopus Laevis);
- Gene ID
- 27122
- UniProt ID
- Q9UBP4
- Long Name
- Dickkopf WNT Signaling Pathway Inhibitor 3
- Sequence Similarities
- Belongs to the dickkopf family.
- Cellular Localization
- Secreted
- Protein Refseq
- XP_006718241.1; XP_016873044.1; XP_047282730.1; XP_047282731.1; NP_001018067.1
- Function
- Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).
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Related Signaling Pathways
Canonical Wnt Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "DKK3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0055CT | Recombinant Mouse anti-Human DKK3 Monoclonal antibody (5H8) | ELISA, FC, WB | |
| HPAB-2833LY | Mouse Anti-DKK3 Recombinant Antibody (HPAB-2833LY) | ELISA, FC, Block | Mouse IgG |
| VS3-QX326 | Mouse Anti-DKK3 Recombinant Antibody (clone 4G7) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-XY580 | Mouse Anti-DKK3 Recombinant Antibody (clone 8A5C6) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS3-FY1698 | Rabbit Anti-DKK3 Recombinant Antibody (clone R03-8G7) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4138 | Hi-Affi™ Rabbit Anti-Dkk3 Recombinant Antibody (clone SI211DS) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2833LY-S(P) | Mouse Anti-DKK3 Recombinant Antibody; scFv Fragment (HPAB-2833LY-S(P)) | ELISA, FC, Block | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2833LY-F(E) | Mouse Anti-DKK3 Recombinant Antibody; Fab Fragment (HPAB-2833LY-F(E)) | ELISA, FC, Block | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY166 | Mouse Anti-NHP DKK3 Recombinant Antibody (clone 4G7) | WB, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY668 | Anti-DKK3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2004 | Anti-Human DKK3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC91 | Recombinant Anti-DKK3 Eliminating Antibody, pH-Sensitive (VS-0625-YC91) | Antigen-Sweeping In Vivo. | Mouse IgG |
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