Mouse Anti-DLAT Recombinant Antibody
CAT#: VS-0725-YC82
The antibody reacts with human and Mouse DLAT protein.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40786/754_D6_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/62766/1191_F12_2_selected.jpg
Subcellular Location
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach, lower shows strong granular cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40786/ihc_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3782/671_G12_1_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3782/11061_B_8_5.jpg
Normal Tissue
Figure 6 Oral mucosa
Squamous epithelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40786/125165_A_7_1.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40786/125165_A_9_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40786/125165_A_9_4.jpg
Normal Tissue
Figure 9 Testis
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40786/125165_A_9_5.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3782/11061_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000150768-DLAT
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Specifications
- Immunogen
- Recombinant human DLAT protein.
- Host Species
- Mouse
- Type
- Mouse IgG
- Species Reactivity
- Human, Mouse
- Applications
- ICC, IF, IP, WB
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 0.1M Tris-Glycine (pH 7.4), 0.15 M NaCl and 50% Glycerol
- Preservative
- 0.2% Sodium azide
- Storage
- Store at 4°C for one week. Store at -20°C for long-term storage. Avoid repeated freeze / thaw cycles.
Applications
- Application Notes
- Immunocytochemistry: 1/300
Immunofluorescence: 1/300
Western Blot: 1/1000
Target
- Alternative Names
- Dihydrolipoamide S-Acetyltransferase; Dihydrolipoamide Acetyltransferase Component Of Pyruvate Dehydrogenase Complex; 70 KDa Mitochondrial Autoantigen Of Primary Biliary Cirrhosis; E2 Component Of Pyruvate Dehydrogenase Complex; Pyruvate Dehydrogenase Complex Component E2; M2 Antigen Complex 70 KDa Subunit; EC 2.3.1.12;
- Gene ID
- 1737
- UniProt ID
- P10515
- Long Name
- Dihydrolipoamide S-Acetyltransferase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| MOB-1120z | Mouse Anti-DLAT Recombinant Antibody (clone 19C3) | WB, ICC, IF, IP | Mouse IgG1 |
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| VS13-YC305 | Rabbit Anti-DLAT Recombinant Antibody (VS13-YC305) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0160MZ | Chicken Anti-DLAT Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0993 | Hi-Affi™ Rabbit Anti-DLAT Recombinant Antibody (clone DS993AB) | WB, FC, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| VS-0724-YC639 | AbPlus™ Anti-DLAT Magnetic Beads (VS-0724-YC639) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
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| VS-0325-XY669 | Anti-DLAT Immunohistochemistry Kit | IHC | |
| VS-0525-XY2005 | Anti-Human DLAT Immunohistochemistry Kit | IHC |
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