Human Anti-DLL4 Recombinant Antibody (clone 61B) (CAT#: HPAB-1108-FY)

This product is a recombinant antibody that recognizes DLL4. The monoclonal antibody 61B reacts with the epitope of DLL4.


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ELISA

Figure 1 61B has no cross-reactivity to mouse Dll4.

Figure 1 61B has no cross-reactivity to mouse Dll4.

The bound AP fusion proteins were detected using AP substrate and a plate reader. Experiment was performed in triplicate. Data are presented as mean ±SEM.

Zhou, B., Wang, H., Liu, R., Wang, M., Deng, H., Giglio, B. C., ... & Li, Z. (2015). PET Imaging of Dll4 Expression in Glioblastoma and Colorectal Cancer Xenografts Using 64Cu-Labeled Monoclonal Antibody 61B. Molecular pharmaceutics, 12(10), 3527-3534.

IF

Figure 2 Confocal fluorescence images of 61B-Cy5.5 and immunofluorescence staining of CD31 in HT29 tumor tissues.

Figure 2 Confocal fluorescence images of 61B-Cy5.5 and immunofluorescence staining of CD31 in HT29 tumor tissues.

Images were obtained under identical conditions and displayed at same magnification. Scale bar = 100 μm.

Zhou, B., Wang, H., Liu, R., Wang, M., Deng, H., Giglio, B. C., ... & Li, Z. (2015). PET Imaging of Dll4 Expression in Glioblastoma and Colorectal Cancer Xenografts Using 64Cu-Labeled Monoclonal Antibody 61B. Molecular pharmaceutics, 12(10), 3527-3534.

IF

Figure 3 IF result of 61B antibody.

Figure 3 IF result of 61B antibody.

61B-Alexa Fluor555 staining of U87MG cells in the absence (upper panel) and presence (lower panel) of excess amount of un-labeled 61B antibody.

Zhou, B., Wang, H., Liu, R., Wang, M., Deng, H., Giglio, B. C., ... & Li, Z. (2015). PET Imaging of Dll4 Expression in Glioblastoma and Colorectal Cancer Xenografts Using 64Cu-Labeled Monoclonal Antibody 61B. Molecular pharmaceutics, 12(10), 3527-3534.


Specifications

  • Host Species
  • Human
  • Type
  • Human IgG
  • Specificity
  • Human DLL4
  • Species Reactivity
  • Human
  • Clone
  • 61B
  • Applications
  • ELISA

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The DLL4 antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Immunofluorescence.

Target

  • Alternative Names
  • Delta Like Canonical Notch Ligand 4; Drosophila Delta Homolog 4; Delta4; Delta-Like 4 Homolog (Drosophila); Delta-Like 4 (Drosophila); Delta-Like 4 Homolog; Delta-Like 4 Protein; Delta-Like Protein 4

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone 61B"

See other products for "DLL4"

Humanized Antibody

CAT Product Name Application Type
TAB-204 Anti-Human DLL4 Recombinant Antibody (Demcizumab) ELISA, FC, IP, FuncS, IF, Neut, WB IgG2 - kappa

Rat Antibody

CAT Product Name Application Type
TAB-217CL Human Anti-DLL4 Recombinant Antibody (TAB-217CL) WB, IHC, IF, FC, Inhib Human IgG

Human Antibody

Blocking Antibody

CAT Product Name Application Type
NEUT-707CQ Hamster Anti-Dll4 Recombinant Antibody (clone HMD4-2) Block Hamster IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-4140 Hi-Affi™ Rabbit Anti-DLL4 Recombinant Antibody (clone SI213DS) ELISA Rabbit IgG

Recombinant Antibody

CAT Product Name Application Type
HPAB-0203-CN Human Anti-DLL4 Recombinant Antibody (HPAB-0203-CN) ELISA, WB, FC, IP Human IgG1
HPAB-0204-CN Human Anti-DLL4 Recombinant Antibody (HPAB-0204-CN) ELISA, WB, FC, IP Human IgG1
HPAB-0205-CN Human Anti-DLL4 Recombinant Antibody (HPAB-0205-CN) ELISA, WB, FC, IP Human IgG4
HPAB-0206-CN Mouse Anti-DLL4 Recombinant Antibody (HPAB-0206-CN) ELISA, WB, FC, IHC, IF, ICC Mouse IgG2
HPAB-0208-CN Human Anti-DLL4 Recombinant Antibody (HPAB-0208-CN) ELISA, WB, FC, IHC, IF, ICC Humanized IgG2

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-204 Afuco™ Anti-DLL4 ADCC Recombinant Antibody (Demcizumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody

Single-domain Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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