Mouse Anti-DNA Recombinant Antibody (clone PL9-11) (CAT#: FAMAB-0090YC)

Provided is monoclonal antibody PL9-11 against DNA. The clone exhibits significant binding to DNA in the absence of histones.

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ELISA

Figure 1 Binding specificities of group A mAbs.

Figure 1 Binding specificities of group A mAbs.

ELISA plates were coated with either H2A-H2B dimers or poly-L-lysine. For the detection of antibodies to DNA or to H2A-H2B-DNA complexes, DNA was added to plates already coated with poly-Llysine or with H2A-H2B dimers.respectively (cf. Methods).

IF

Figure 2 Immunofluorescence staining of HEp-2 cells by PL9-2 mAbs.

Figure 2 Immunofluorescence staining of HEp-2 cells by PL9-2 mAbs.

PL9-3, PL9-9, PL9-7, and LG4-3 gave a pattern similar to PL9-2, whereas PL9-10 and PL9-11 were similar to PL9-6 (not shown). The mAbs were used at 20 ug/ml in PBS-BSA.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG3, κ
  • Specificity
  • Mouse DNA
  • Species Reactivity
  • Mouse
  • Clone
  • PL9-11
  • Applications
  • ELISA, IF

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • Deoxyribonucleic acid; DNA

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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