Rabbit Anti-DOCK8 Recombinant Antibody (clone R05-7D3) (CAT#: VS3-FY1701)

• Immunogen
• Recombinant human DOCK8 protein

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human DOCK8

• Species Reactivity
• Human

• Clone
• R05-7D3

• Applications
• Western Blot, Immunofluorescence

• MW
• The calculated weight of the target protein is approximately 239 kDa, while the observed molecular weight stands at 239 kDa.

• Related Disease
• Hyper-Ige Syndrome 2, Autosomal Recessive, With Recurrent Infections and Combined Immunodeficiency Due To Dock8 Deficiency

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunofluorescence: 1/50-1/200

• Alternative Names
• Dedicator Of Cytokinesis 8; Dedicator Of Cytokinesis Protein 8; Epididymis Luminal Protein 205; 1200017A24Rik; HEL-205; MRD2; ZIR8

• Gene ID
• 81704

• UniProt ID
• Q8NF50

• Sequence Similarities
• Belongs to the DOCK family.

• Cellular Localization
• Cell membrane, Cell projection, Cytoplasm, Membrane

• Post Translation Modifications
• In response to chemokine CXCL12/SDF-1-alpha stimulation, phosphorylated by PRKCA/PKC-alpha which promotes DOCK8 dissociation from LRCH1.

• Protein Refseq
• XP_011516347.1; XP_011516348.1; XP_016870662.1; XP_047279883.1; XP_047279884.1

• Function
• Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP.
  During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC (By similarity).
  Required for CD4⁺ T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane.
  Is involved in NK cell cytotoxicity by controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody