Rabbit Anti-DOCK8 Recombinant Antibody (clone R05-7D3)
CAT#: VS3-FY1701
Clone R05-7D3 is a rabbit IgG recombinant monoclonal antibody that is designed for the specific detection of human DOCK8. The immunogen utilized in its development is a recombinant protein of human DOCK8. The antibody has a calculated molecular weight of 239 kDa and an observed molecular weight of 239 kDa.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R05-7D3 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunofluorescence (dilution range 1:50-1:200).
Specifications
- Immunogen
- Recombinant human DOCK8 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human DOCK8
- Species Reactivity
- Human
- Clone
- R05-7D3
- Applications
- Western Blot, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 239 kDa, while the observed molecular weight stands at 239 kDa.
- Related Disease
- Hyper-Ige Syndrome 2, Autosomal Recessive, With Recurrent Infections and Combined Immunodeficiency Due To Dock8 Deficiency
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Dedicator Of Cytokinesis 8; Dedicator Of Cytokinesis Protein 8; Epididymis Luminal Protein 205; 1200017A24Rik; HEL-205; MRD2; ZIR8
- Gene ID
- 81704
- UniProt ID
- Q8NF50
- Sequence Similarities
- Belongs to the DOCK family.
- Cellular Localization
- Cell membrane, Cell projection, Cytoplasm, Membrane
- Post Translation Modifications
- In response to chemokine CXCL12/SDF-1-alpha stimulation, phosphorylated by PRKCA/PKC-alpha which promotes DOCK8 dissociation from LRCH1.
- Protein Refseq
- XP_011516347.1; XP_011516348.1; XP_016870662.1; XP_047279883.1; XP_047279884.1
- Function
- Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP.
During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC (By similarity).
Required for CD4⁺ T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane.
Is involved in NK cell cytotoxicity by controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "DOCK8"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2054 | Anti-DOCK8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2055 | Anti-Mouse DOCK8 Immunohistochemistry Kit | IHC |
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