Mouse Anti-dsDNA Recombinant Antibody (clone G2-6) (CAT#: FAMAB-0110CQ)
Autoantibody
This product is a recombinant mouse antibody clone G2-6, which specifically binds to dsDNA.
Specific Inquiry
WB
Figure 1 Analysis of G2-6 scFv by Western blotting
Soluble extract and scFv purified using the histidine affinity column for His-tag were visualized by Western blotting.
Im, S. W., Chung, H. Y., & Jang, Y. J. (2017). Development of single-chain Fv of antibody to DNA as intracellular delivery vehicle. Animal Cells and Systems, 21(6), 382-387.
ELISA
Figure 2 Analysis of G2-6 scFv by DNA-binding ELISA
The binding affinity of scFv and IgG of G2-6 were analyzed by dsDNA-binding ELISA. Data are presented as the mean ± S.D. from triplicate experiments.
Im, S. W., Chung, H. Y., & Jang, Y. J. (2017). Development of single-chain Fv of antibody to DNA as intracellular delivery vehicle. Animal Cells and Systems, 21(6), 382-387.
FC
Figure 3 Internalization of G2-6 scFv was analyzed by flow cytometry
L929, COS-7, and NIH/3T3 cell lines were incubated with various concentrations of scFv for 1 h, and analyzed by flow cytometry.
Im, S. W., Chung, H. Y., & Jang, Y. J. (2017). Development of single-chain Fv of antibody to DNA as intracellular delivery vehicle. Animal Cells and Systems, 21(6), 382-387.
IF
Figure 4 Internalization of G2-6 scFv was analyzed by confocal microscope
L929 cells were incubated with various concentrations of scFv at for 1 h and analyzed by confocal microscope.
Im, S. W., Chung, H. Y., & Jang, Y. J. (2017). Development of single-chain Fv of antibody to DNA as intracellular delivery vehicle. Animal Cells and Systems, 21(6), 382-387.
IF
Figure 5 Internalization of G2-6 scFv was analyzed by confocal microscope
L929 cells were incubated with 100 µg/ml scFv for various period of time and analyzed by confocal microscope.
Im, S. W., Chung, H. Y., & Jang, Y. J. (2017). Development of single-chain Fv of antibody to DNA as intracellular delivery vehicle. Animal Cells and Systems, 21(6), 382-387.
IF
Figure 6 Internalization of G2-6 scFv was analyzed by confocal microscope
L929 cells were incubated with 150 μg/ml scFv for 1 h, and Z-stack analysis of the image was performed.
Im, S. W., Chung, H. Y., & Jang, Y. J. (2017). Development of single-chain Fv of antibody to DNA as intracellular delivery vehicle. Animal Cells and Systems, 21(6), 382-387.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Clone
- G2-6
- Applications
- IF, IHC
- Related Disease
- Systemic lupus erythematosus (SLE)
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunofluorescence, Enzyme-linked Immunosorbent Assay, Western Blot and Flow Cytometry.
Target
- Alternative Names
- DsDNA; double-stranded DNA
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone G2-6"
See other products for "dsDNA"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
IgM Antibody Products
Fab Antibody Products
ScFv Antibody Products
Autoantibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-077 | Mouse Anti-dsDNA Recombinant Antibody (clone 64M-5) | ELISA, WB, FuncS | Mouse IgG |
| FAMAB-0108CQ | Mouse Anti-dsDNA Recombinant Antibody (clone 17-45) | ELISA | Mouse IgG2a |
| HPAB-0996WJ | Mouse Anti-dsDNA Recombinant Antibody (clone Homl2H5) | ELISA | Mouse IgM |
| HPAB-0997WJ | Mouse Anti-dsDNA Recombinant Antibody (clone Homl3D2) | ELISA | Mouse IgM |
| HPAB-0998WJ | Mouse Anti-dsDNA Recombinant Antibody (clone Homl5G9) | ELISA | Mouse IgM |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for FAMAB-0110CQ. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: WB, ELISA, IP, FC, FuncS, Neut, IF
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: ELISA, Neut, FuncS
-1.jpg)
-1-1.png)
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
