Mouse Anti-dsDNA Recombinant Antibody (clone G2-6) (CAT#: FAMAB-0110CQ)
This product is a recombinant mouse antibody clone G2-6, which specifically binds to dsDNA.
Specific Inquiry
WB
Figure 1 Analysis of G2-6 scFv by Western blotting
Soluble extract and scFv purified using the histidine affinity column for His-tag were visualized by Western blotting.
Im, S. W., Chung, H. Y., & Jang, Y. J. (2017). Development of single-chain Fv of antibody to DNA as intracellular delivery vehicle. Animal Cells and Systems, 21(6), 382-387.
ELISA
Figure 2 Analysis of G2-6 scFv by DNA-binding ELISA
The binding affinity of scFv and IgG of G2-6 were analyzed by dsDNA-binding ELISA. Data are presented as the mean ± S.D. from triplicate experiments.
Im, S. W., Chung, H. Y., & Jang, Y. J. (2017). Development of single-chain Fv of antibody to DNA as intracellular delivery vehicle. Animal Cells and Systems, 21(6), 382-387.
FC
Figure 3 Internalization of G2-6 scFv was analyzed by flow cytometry
L929, COS-7, and NIH/3T3 cell lines were incubated with various concentrations of scFv for 1 h, and analyzed by flow cytometry.
Im, S. W., Chung, H. Y., & Jang, Y. J. (2017). Development of single-chain Fv of antibody to DNA as intracellular delivery vehicle. Animal Cells and Systems, 21(6), 382-387.
IF
Figure 4 Internalization of G2-6 scFv was analyzed by confocal microscope
L929 cells were incubated with various concentrations of scFv at for 1 h and analyzed by confocal microscope.
Im, S. W., Chung, H. Y., & Jang, Y. J. (2017). Development of single-chain Fv of antibody to DNA as intracellular delivery vehicle. Animal Cells and Systems, 21(6), 382-387.
IF
Figure 5 Internalization of G2-6 scFv was analyzed by confocal microscope
L929 cells were incubated with 100 µg/ml scFv for various period of time and analyzed by confocal microscope.
Im, S. W., Chung, H. Y., & Jang, Y. J. (2017). Development of single-chain Fv of antibody to DNA as intracellular delivery vehicle. Animal Cells and Systems, 21(6), 382-387.
IF
Figure 6 Internalization of G2-6 scFv was analyzed by confocal microscope
L929 cells were incubated with 150 μg/ml scFv for 1 h, and Z-stack analysis of the image was performed.
Im, S. W., Chung, H. Y., & Jang, Y. J. (2017). Development of single-chain Fv of antibody to DNA as intracellular delivery vehicle. Animal Cells and Systems, 21(6), 382-387.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Clone
- G2-6
- Applications
- IF, IHC
- Related Disease
- Systemic lupus erythematosus (SLE)
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunofluorescence, Enzyme-linked Immunosorbent Assay, Western Blot and Flow Cytometry.
Target
- Alternative Names
- DsDNA; double-stranded DNA
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone G2-6"
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scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0092-YC-S(P) | Mouse Anti-dsDNA Recombinant Antibody (clone 165.60); scFv Fragment | ELISA | Mouse scFv |
| FAMAB-0093-YC-S(P) | Mouse Anti-dsDNA Recombinant Antibody (clone 163.72); scFv Fragment | ELISA | Mouse scFv |
| FAMAB-0047WJ-S(P) | Human Anti-dsDNA Recombinant Antibody (clone T14); scFv Fragment | WB | Human scFv |
| FAMAB-0064WJ-S(P) | Human Anti-dsDNA Recombinant Antibody (clone KSUN); scFv Fragment | ELISA | Human scFv |
| FAMAB-0058JF-S(P) | Human Anti-dsDNA Recombinant Antibody (clone A6); scFv Fragment | FC, WB, IHC, ELISA, FuncS | Human scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0092-YC-F(E) | Mouse Anti-dsDNA Recombinant Antibody (clone 165.60); Fab Fragment | ELISA | Mouse Fab |
| FAMAB-0093-YC-F(E) | Mouse Anti-dsDNA Recombinant Antibody (clone 163.72); Fab Fragment | ELISA | Mouse Fab |
| HPAB-0996WJ-F(E) | Mouse Anti-dsDNA Recombinant Antibody (clone Homl2H5); Fab Fragment | ELISA | Mouse Fab |
| HPAB-0997WJ-F(E) | Mouse Anti-dsDNA Recombinant Antibody (clone Homl3D2); Fab Fragment | ELISA | Mouse Fab |
| HPAB-0998WJ-F(E) | Mouse Anti-dsDNA Recombinant Antibody (clone Homl5G9); Fab Fragment | ELISA | Mouse Fab |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0108CQ | Mouse Anti-dsDNA Recombinant Antibody (clone 17-45) | ELISA | Mouse IgG2a |
| FAMAB-0109CQ | Mouse Anti-dsDNA Recombinant Antibody (clone 2C10) | ELISA | Mouse IgG |
| FAMAB-0013WJ | Human Anti-dsDNA Recombinant Antibody (clone RH-14) | ELISA | Human IgG1, λ |
| FAMAB-0014WJ | Human Anti-dsDNA Recombinant Antibody (clone DIL-6) | ELISA | Human IgG3, λ |
| FAMAB-0151JF | Mouse Anti-dsDNA Recombinant Antibody (clone G6-7) | WB, ELISA, IF, FC, FuncS | Mouse IgG2b |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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