Human Anti-DSG1 Recombinant Antibody (clone (D31)2/28) (CAT#: FAMAB-0206-CN)

Figure 1 IF of (D31)2/28 scFv mAbs on human and mouse epidermis.

The ScFv mAb used as primary antibody for staining human and mouse epidermis is indicated in each panel. Magnification, ×400.

Payne, A. S., Ishii, K., Kacir, S., Lin, C., Li, H., Hanakawa, Y., ... & Siegel, D. L. (2005). Genetic and functional characterization of human pemphigus vulgaris monoclonal autoantibodies isolated by phage display. The Journal of clinical investigation, 115(4), 888-899

Figure 2 Characterization of scFv mAb desmoglein-binding specificity by ELISA.

Patterns of the binding of selected scFv mAb clones to Dsg1 and Dsg3.

Payne, A. S., Ishii, K., Kacir, S., Lin, C., Li, H., Hanakawa, Y., ... & Siegel, D. L. (2005). Genetic and functional characterization of human pemphigus vulgaris monoclonal autoantibodies isolated by phage display. The Journal of clinical investigation, 115(4), 888-899.

Figure 3 Epitopes recognized by PV scFv mAbs are blocked by sera from multiple pemphigus patients.

(D31)2/28. Left: Inhibition of Dsg3binding by 7 of 8 PV sera and 0 of 6 PF sera. Right: Inhibition of Dsg1 binding by 4 of 5 PV(3+1) sera and 5 of 6 PF sera. Inhibition of greater than 20% was considered positive. NHS, normal human sera.

Payne, A. S., Ishii, K., Kacir, S., Lin, C., Li, H., Hanakawa, Y., ... & Siegel, D. L. (2005). Genetic and functional characterization of human pemphigus vulgaris monoclonal autoantibodies isolated by phage display. The Journal of clinical investigation, 115(4), 888-899

Figure 4 Epitope mapping of (D31)2/28 against mouse and human desmogleins.

Wild-type and domain-swapped extracellular domains of mDsg1 (lanes 1–6) or wild-type extracellular domains of human desmogleins (hDsgs) 1, 3, and 4 (lanes 7–9) were produced in baculovirus and immunoprecipitated with scFv mAbs. Desmoglein proteins bound by human scFv mAbs were detected by immunoblot analysis using antibody against E tag, which was engineered onto the carboxyterminal domain of recombinant desmoglein molecules. The top panel shows the immunoblot of the recombinant and chimeric desmogleins detected with anti–E tag. The bottom 2 panels show the results of the immunoprecipitation-immunoblotting studies.

Payne, A. S., Ishii, K., Kacir, S., Lin, C., Li, H., Hanakawa, Y., ... & Siegel, D. L. (2005). Genetic and functional characterization of human pemphigus vulgaris monoclonal autoantibodies isolated by phage display. The Journal of clinical investigation, 115(4), 888-899