Human Anti-DSG3 Recombinant Antibody (clone (D3)3c/9) (CAT#: FAMAB-0204-CN)

This is a human pemphigus vulgaris monoclonal autoantibodies isolated by phage display. The clone (D3)3c/9, although selected for binding against only hDsg3, also demonstrates weak binding to hDsg1. Howerver, (D3)3c/9 shows different binding pattern on mouse epidermis, which recognizes only mDsg3 and not mDsg1.


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IF

Figure 1 IF of (D3)3c/9 scFv mAbs on human and mouse epidermis.

Figure 1 IF of (D3)3c/9 scFv mAbs on human and mouse epidermis.

The ScFv mAb used as primary antibody for staining human and mouse epidermis is indicated in each panel. Magnification, ×400.

Payne, A. S., Ishii, K., Kacir, S., Lin, C., Li, H., Hanakawa, Y., ... & Siegel, D. L. (2005). Genetic and functional characterization of human pemphigus vulgaris monoclonal autoantibodies isolated by phage display. The Journal of clinical investigation, 115(4), 888-899

ELISA

Figure 2 Characterization of scFv mAb desmoglein-binding specificity by ELISA.

Figure 2 Characterization of scFv mAb desmoglein-binding specificity by ELISA.

(D3)3c/9 was isolated from a library panned on Dsg3 but showed quantifiable specificity for Dsg1.

Payne, A. S., Ishii, K., Kacir, S., Lin, C., Li, H., Hanakawa, Y., ... & Siegel, D. L. (2005). Genetic and functional characterization of human pemphigus vulgaris monoclonal autoantibodies isolated by phage display. The Journal of clinical investigation, 115(4), 888-899

Inhib

Figure 3 Epitopes recognized by PV scFv mAbs are blocked by sera from multiple pemphigus patients.

Figure 3 Epitopes recognized by PV scFv mAbs are blocked by sera from multiple pemphigus patients.

(D3)3c/9. Left: Inhibition of Dsg3 binding by all 8 PV sera. PVLIB is the plasma of the mucocutaneous PV patient from whom the phage display library was made. Black bars represent PV sera containing anti-Dsg3 antibodies only; gray bars indicate PV sera containing antibodies with both anti-Dsg3 and anti-Dsg1 activity [PV(3+1) sera]. Right: Inhibition of Dsg1 binding by 3 of 5 PV(3+1) sera and 6 of 6 PF sera (white bars).

Payne, A. S., Ishii, K., Kacir, S., Lin, C., Li, H., Hanakawa, Y., ... & Siegel, D. L. (2005). Genetic and functional characterization of human pemphigus vulgaris monoclonal autoantibodies isolated by phage display. The Journal of clinical investigation, 115(4), 888-899

IP

Figure 4 Epitope mapping of (D3)3c/9 against mouse and human desmogleins.

Figure 4 Epitope mapping of (D3)3c/9 against mouse and human desmogleins.

Wild-type and domain-swapped extracellular domains of mDsg3 (lanes 1–6) or wild-type extracellular domains of human desmogleins (hDsgs) 1, 3, and 4 (lanes 7–9) were produced in baculovirus and immunoprecipitated with scFv mAbs. Desmoglein proteins bound by human scFv mAbs were detected by immunoblot analysis using antibody against E tag, which was engineered onto the carboxyterminal domain of recombinant desmoglein molecules. The top panel shows the immunoblot of the recombinant and chimeric desmogleins detected with anti–E tag. The bottom 2 panels show the results of the immunoprecipitation-immunoblotting studies.

Payne, A. S., Ishii, K., Kacir, S., Lin, C., Li, H., Hanakawa, Y., ... & Siegel, D. L. (2005). Genetic and functional characterization of human pemphigus vulgaris monoclonal autoantibodies isolated by phage display. The Journal of clinical investigation, 115(4), 888-899

IP

Figure 5 PV scFv mAbs recognize primarily but not exclusively conformational epitopes on desmogleins.

Figure 5 PV scFv mAbs recognize primarily but not exclusively conformational epitopes on desmogleins.

Immunoblot analysis of human keratinocyte extract using (D3)3c/9. Lane 1, Coomassie blue–stained human keratinocyte extract. Lane 2, murine anti-Dsg3 mAb 5G11. Lane 3, (D3)3c/9. Lane 4, E1M2 (control anti–human red blood cell scFv).

Payne, A. S., Ishii, K., Kacir, S., Lin, C., Li, H., Hanakawa, Y., ... & Siegel, D. L. (2005). Genetic and functional characterization of human pemphigus vulgaris monoclonal autoantibodies isolated by phage display. The Journal of clinical investigation, 115(4), 888-899


Specifications

  • Host Species
  • Human
  • Derivation
  • Phage display library
  • Type
  • Human IgG
  • Specificity
  • Human DSG3
  • Species Reactivity
  • Human, Mouse
  • Clone
  • (D3)3c/9
  • Applications
  • ELISA, IF, IB
  • Related Disease
  • Pemphigus

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been reported for use in Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunoblot, Inhibition and Immunoprecipitation.

Target

  • Alternative Names
  • Desmoglein 3; 130 KDa Pemphigus Vulgaris Antigen; Cadherin Family Member 6; CDHF6; PVA; Desmoglein 3 (Pemphigus Vulgaris Antigen); Pemphigus Vulgaris Antigen

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1055 Hi-Affi™ Rabbit Anti-DSG3 Recombinant Antibody (clone DS1055AB) WB Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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