This is a human pemphigus vulgaris monoclonal autoantibodies isolated by phage display. The clone (D3)3c/9, although selected for binding against only hDsg3, also demonstrates weak binding to hDsg1. Howerver, (D3)3c/9 shows different binding pattern on mouse epidermis, which recognizes only mDsg3 and not mDsg1.
Figure 1 IF of (D3)3c/9 scFv mAbs on human and mouse epidermis.
The ScFv mAb used as primary antibody for staining human and mouse epidermis is indicated in each panel. Magnification, ×400.
Payne, A. S., Ishii, K., Kacir, S., Lin, C., Li, H., Hanakawa, Y., ... & Siegel, D. L. (2005). Genetic and functional characterization of human pemphigus vulgaris monoclonal autoantibodies isolated by phage display. The Journal of clinical investigation, 115(4), 888-899
Figure 2 Characterization of scFv mAb desmoglein-binding specificity by ELISA.
(D3)3c/9 was isolated from a library panned on Dsg3 but showed quantifiable specificity for Dsg1.
Payne, A. S., Ishii, K., Kacir, S., Lin, C., Li, H., Hanakawa, Y., ... & Siegel, D. L. (2005). Genetic and functional characterization of human pemphigus vulgaris monoclonal autoantibodies isolated by phage display. The Journal of clinical investigation, 115(4), 888-899
Figure 3 Epitopes recognized by PV scFv mAbs are blocked by sera from multiple pemphigus patients.
(D3)3c/9. Left: Inhibition of Dsg3 binding by all 8 PV sera. PVLIB is the plasma of the mucocutaneous PV patient from whom the phage display library was made. Black bars represent PV sera containing anti-Dsg3 antibodies only; gray bars indicate PV sera containing antibodies with both anti-Dsg3 and anti-Dsg1 activity [PV(3+1) sera]. Right: Inhibition of Dsg1 binding by 3 of 5 PV(3+1) sera and 6 of 6 PF sera (white bars).
Payne, A. S., Ishii, K., Kacir, S., Lin, C., Li, H., Hanakawa, Y., ... & Siegel, D. L. (2005). Genetic and functional characterization of human pemphigus vulgaris monoclonal autoantibodies isolated by phage display. The Journal of clinical investigation, 115(4), 888-899
Figure 4 Epitope mapping of (D3)3c/9 against mouse and human desmogleins.
Wild-type and domain-swapped extracellular domains of mDsg3 (lanes 1–6) or wild-type extracellular domains of human desmogleins (hDsgs) 1, 3, and 4 (lanes 7–9) were produced in baculovirus and immunoprecipitated with scFv mAbs. Desmoglein proteins bound by human scFv mAbs were detected by immunoblot analysis using antibody against E tag, which was engineered onto the carboxyterminal domain of recombinant desmoglein molecules. The top panel shows the immunoblot of the recombinant and chimeric desmogleins detected with anti–E tag. The bottom 2 panels show the results of the immunoprecipitation-immunoblotting studies.
Payne, A. S., Ishii, K., Kacir, S., Lin, C., Li, H., Hanakawa, Y., ... & Siegel, D. L. (2005). Genetic and functional characterization of human pemphigus vulgaris monoclonal autoantibodies isolated by phage display. The Journal of clinical investigation, 115(4), 888-899
Figure 5 PV scFv mAbs recognize primarily but not exclusively conformational epitopes on desmogleins.
Immunoblot analysis of human keratinocyte extract using (D3)3c/9. Lane 1, Coomassie blue–stained human keratinocyte extract. Lane 2, murine anti-Dsg3 mAb 5G11. Lane 3, (D3)3c/9. Lane 4, E1M2 (control anti–human red blood cell scFv).
Payne, A. S., Ishii, K., Kacir, S., Lin, C., Li, H., Hanakawa, Y., ... & Siegel, D. L. (2005). Genetic and functional characterization of human pemphigus vulgaris monoclonal autoantibodies isolated by phage display. The Journal of clinical investigation, 115(4), 888-899
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CAT | Product Name | Application | Type |
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MOB-2196MZ | Recombinant Mouse Anti-Human DSG3 Antibody (clone 4H244) | ICC/IF, IHC-P, IP, WB | Mouse antibody |
FAMAB-0203-CN | Human Anti-DSG3 Recombinant Antibody (clone (D3)1b/3a) | ELISA, IF, IB | Human IgG |
VS3-QX332 | Mouse Anti-DSG3 Recombinant Antibody (clone 1G6A7) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
VS3-QX333 | Mouse Anti-DSG3 Recombinant Antibody (clone 11G-2C) | WB, IHC, FC | Mouse IgG1 |
VS4-WK149 | Mouse Anti-DSG3 Recombinant Antibody (clone 4C2) | ELISA | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1055 | Hi-Affi™ Rabbit Anti-DSG3 Recombinant Antibody (clone DS1055AB) | WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0203-CN-S(P) | Human Anti-DSG3 Recombinant Antibody (clone (D3)1b/3a); scFv Fragment | ELISA, IF, IB | Human scFv |
FAMAB-0204-CN-S(P) | Human Anti-DSG3 Recombinant Antibody (clone (D3);3c/9); scFv Fragment | ELISA, IF, IB | Human scFv |
FAMAB-0205-CN-S(P) | Human Anti-DSG3 Recombinant Antibody (clone (D3)3a/9); scFv Fragment | ELISA, IF, IB | Human scFv |
FAMAB-0152JF-S(P) | Human Anti-DSG3 Recombinant Antibody (clone PVMAB786); scFv Fragment | IF, WB, ELISA, FuncS | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0203-CN-F(E) | Human Anti-DSG3 Recombinant Antibody (clone (D3)1b/3a); Fab Fragment | ELISA, IF, IB | Human Fab |
FAMAB-0204-CN-F(E) | Human Anti-DSG3 Recombinant Antibody (clone (D3)3c/9); Fab Fragment | ELISA, IF, IB | Human Fab |
FAMAB-0205-CN-F(E) | Human Anti-DSG3 Recombinant Antibody (clone (D3)3a/9); Fab Fragment | ELISA, IF, IB | Human Fab |
FAMAB-0152JF-F(E) | Human Anti-DSG3 Recombinant Antibody (clone PVMAB786); Fab Fragment | IF, WB, ELISA, FuncS | Human Fab |
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