Human Anti-DSG3 Recombinant Antibody (clone 3.2) (CAT#: FAMAB-0516WJ)
This product is a human monoclonal antibody that is specific for Human DSG3. This antibody can be used in a variety of applications, such as ELISA, WB, IF.
Specific Inquiry
IF
Figure 1 Indirect IF staining of human skin with scFv mAbs demonstrates cell surface binding typical of PV.
Negative control is an scFv mAb against an irrelevant Ag. Positive control is a previously characterized anti-Dsg3 scFv mAb, (D31)2/2918. Scale bar, 20 μM.
Cho, M. J., Lo, A. S., Mao, X., Nagler, A. R., Ellebrecht, C. T., Mukherjee, E. M., ... & Payne, A. S. (2014). Shared VH1-46 gene usage by pemphigus vulgaris autoantibodies indicates common humoral immune responses among patients. Nature communications, 5(1), 1-11.
Figure 2 VH1-46 mAbs, but not VH5a mAb, induce suprabasal skin blisters in human skin organ culture. Scale bar, 100 μM.
Cho, M. J., Lo, A. S., Mao, X., Nagler, A. R., Ellebrecht, C. T., Mukherjee, E. M., ... & Payne, A. S. (2014). Shared VH1-46 gene usage by pemphigus vulgaris autoantibodies indicates common humoral immune responses among patients. Nature communications, 5(1), 1-11.
ELISA
Figure 3 GL LC/HC recombinant Abs were generated for those mAbs that were insoluble in their fully GL state.
(a) F706 germline-reverted (GL) and (b) F779GL Abs do not bind Dsg3. (c) 3.2 GL Ab binds Dsg3 by ELISA with reduced affinity. (d) PVE4-8 GL and (e) 4.2 GL Abs retain binding to Dsg3 by ELISA. Black/grey hatched lines indicate GL1/2 Abs, respectively. (f-j) Germline-reverted non-VH1-46 mAbs do not demonstrate binding to Dsg3 by ELISA.
Cho, M. J., Lo, A. S., Mao, X., Nagler, A. R., Ellebrecht, C. T., Mukherjee, E. M., ... & Payne, A. S. (2014). Shared VH1-46 gene usage by pemphigus vulgaris autoantibodies indicates common humoral immune responses among patients. Nature communications, 5(1), 1-11.
IF
Figure 4 Indirect IF (IIF) staining of human skin confirms Dsg3 autoreactivity of PVE4-8 GL and 4.2 GL Abs in native skin tissue.
IIF binding of 3.2 GL was not detectable, presumably due to low affinity. Scale bar, 20 μM. Error bars indicate s.e.m. Data are representative of three to five independent experiments.
Cho, M. J., Lo, A. S., Mao, X., Nagler, A. R., Ellebrecht, C. T., Mukherjee, E. M., ... & Payne, A. S. (2014). Shared VH1-46 gene usage by pemphigus vulgaris autoantibodies indicates common humoral immune responses among patients. Nature communications, 5(1), 1-11.
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human DSG3
- Species Reactivity
- Human
- Clone
- 3.2
- Applications
- ELISA, WB, IF
- Related Disease
- Pemphigus vulgaris
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "DSG3"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
Mouse Antibody Products
Rabbit Monoclonal Antibody Products
ScFv Antibody Products
Fab Antibody Products
ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-172 | Human Anti-DSG3 Recombinant Antibody (clone PVA3) | ELISA | Human IgG |
| MOB-2196MZ | Recombinant Mouse Anti-Human DSG3 Antibody (clone 4H244) | ICC/IF, IHC-P, IP, WB | Mouse antibody |
| VS3-QX332 | Mouse Anti-DSG3 Recombinant Antibody (clone 1G6A7) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-QX333 | Mouse Anti-DSG3 Recombinant Antibody (clone 11G-2C) | WB, IHC, FC | Mouse IgG1 |
| VS3-XY593 | Mouse Anti-DSG3 Recombinant Antibody (clone 6G2C11) | ELISA, WB, IHC, FC | Mouse IgG1 |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for FAMAB-0516WJ. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: FC, Cyt, Stim, PP, Agonist
Application: WB, IF, IP, Neut, FuncS, ELISA, FC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, Neut, ELISA, IF, IP, FuncS, FC
Application: ELISA, SPR, Inhib, FuncS
Application: ELISA, FuncS, IHC, IF, FC, ADCC
Application: WB, ELISA, FC, IHC, IF, IP
Application: ELISA
Application: ELISA, FC, FuncS
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
