Human Anti-DSG3 Recombinant Antibody (clone F779)
CAT#: FAMAB-0519WJ
This product is a human monoclonal antibody that is specific for Human DSG3. This antibody can be used in a variety of applications, such as ELISA, WB, IF.
Gene Expression
Published Data
Normal Tissue
Figure 1 Esophagus
Squamous epithelial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/56863/148897_B_5_1.jpg
RNA Expression
Figure 2 RNA cell line category: Group enriched (HaCaT, hTCEpi)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000134757-DSG3
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IF
Figure 1 Indirect IF staining of human skin with scFv mAbs demonstrates cell surface binding typical of PV.
Negative control is an scFv mAb against an irrelevant Ag. Positive control is a previously characterized anti-Dsg3 scFv mAb, (D31)2/2918. Scale bar, 20 μM.
Cho, M. J., Lo, A. S., Mao, X., Nagler, A. R., Ellebrecht, C. T., Mukherjee, E. M., ... & Payne, A. S. (2014). Shared VH1-46 gene usage by pemphigus vulgaris autoantibodies indicates common humoral immune responses among patients. Nature communications, 5(1), 1-11.
Figure 2 VH1-46 mAbs, but not VH5a mAb, induce suprabasal skin blisters in human skin organ culture. Scale bar, 100 μM.
Cho, M. J., Lo, A. S., Mao, X., Nagler, A. R., Ellebrecht, C. T., Mukherjee, E. M., ... & Payne, A. S. (2014). Shared VH1-46 gene usage by pemphigus vulgaris autoantibodies indicates common humoral immune responses among patients. Nature communications, 5(1), 1-11.
ELISA
Figure 3 GL LC/HC recombinant Abs were generated for those mAbs that were insoluble in their fully GL state.
(a) F706 germline-reverted (GL) and (b) F779GL Abs do not bind Dsg3. (c) 3.2 GL Ab binds Dsg3 by ELISA with reduced affinity. (d) PVE4-8 GL and (e) 4.2 GL Abs retain binding to Dsg3 by ELISA. Black/grey hatched lines indicate GL1/2 Abs, respectively. (f-j) Germline-reverted non-VH1-46 mAbs do not demonstrate binding to Dsg3 by ELISA.
Cho, M. J., Lo, A. S., Mao, X., Nagler, A. R., Ellebrecht, C. T., Mukherjee, E. M., ... & Payne, A. S. (2014). Shared VH1-46 gene usage by pemphigus vulgaris autoantibodies indicates common humoral immune responses among patients. Nature communications, 5(1), 1-11.
IF
Figure 4 Binding of F779GL1+ D/E to native human skin tissue was confirmed by indirect IF.
Dsg3 autoreactivity of F779SM, F779 germline-reverted (GL) in which all somatic mutations have been removed, F779SM D/E>N/Q in which only two acidic amino-acid residues in the heavy chain CDR2 and light chain CDR1 are mutated to their polar analogues, and F779GL+D/E, in which only the two acidic somatic mutations are reintroduced into the GL Ab.
Cho, M. J., Lo, A. S., Mao, X., Nagler, A. R., Ellebrecht, C. T., Mukherjee, E. M., ... & Payne, A. S. (2014). Shared VH1-46 gene usage by pemphigus vulgaris autoantibodies indicates common humoral immune responses among patients. Nature communications, 5(1), 1-11.
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Specifications
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- Human DSG3
- Species Reactivity
- Human
- Clone
- F779
- Applications
- ELISA, WB, IF
- Related Disease
- Pemphigus vulgaris
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# FAMAB-0519WJ, RRID: AB_3111329)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| TAB-258MZ | Anti-Human DSG3 Recombinant Antibody (TAB-258MZ) | IHC, FC | |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0203-CN-S(P) | Human Anti-DSG3 Recombinant Antibody (clone (D3)1b/3a); scFv Fragment | ELISA, IF, IB | Human scFv |
| FAMAB-0205-CN-S(P) | Human Anti-DSG3 Recombinant Antibody (clone (D3)3a/9); scFv Fragment | ELISA, IF, IB | Human scFv |
| FAMAB-0152JF-S(P) | Human Anti-DSG3 Recombinant Antibody (clone PVMAB786); scFv Fragment | IF, WB, ELISA, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0203-CN-F(E) | Human Anti-DSG3 Recombinant Antibody (clone (D3)1b/3a); Fab Fragment | ELISA, IF, IB | Human Fab |
| FAMAB-0205-CN-F(E) | Human Anti-DSG3 Recombinant Antibody (clone (D3)3a/9); Fab Fragment | ELISA, IF, IB | Human Fab |
| FAMAB-0152JF-F(E) | Human Anti-DSG3 Recombinant Antibody (clone PVMAB786); Fab Fragment | IF, WB, ELISA, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2095 | Anti-DSG3 Immunohistochemistry Kit | IHC | |
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